Typical Standard Curve/Testing Data
Human C type lectin domain family 12 member B (CLEC12B) ELISA Kit | CLEC12B elisa kit
Human C type lectin domain family 12 member B (CLEC12B) ELISA Kit
Gene Names
CLEC12B; UNQ5782
Reactivity
Human
Synonyms
C type lectin domain family 12 member B (CLEC12B); Human C type lectin domain family 12 member B (CLEC12B) ELISA Kit; CLEC12B elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
1.0-25ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for CLEC12B elisa kit
Intended Uses: This CLEC12B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLEC12B. This ELISA kit for research use only!
Principle of the Assay: CLEC12B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC12B antibody and an CLEC12B-HRP conjugate. The assay sample and buffer are incubated together with CLEC12B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC12B concentration since CLEC12B from samples and CLEC12B-HRP conjugate compete for the anti-CLEC12B antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC12B from the sample, fewer sites are left to bind CLEC12B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC12B concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CLEC12B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC12B antibody and an CLEC12B-HRP conjugate. The assay sample and buffer are incubated together with CLEC12B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC12B concentration since CLEC12B from samples and CLEC12B-HRP conjugate compete for the anti-CLEC12B antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC12B from the sample, fewer sites are left to bind CLEC12B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC12B concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CLEC12B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,740 Da
NCBI Official Full Name
C-type lectin domain family 12 member B
NCBI Official Synonym Full Names
C-type lectin domain family 12, member B
NCBI Official Symbol
CLEC12B
NCBI Official Synonym Symbols
UNQ5782
NCBI Protein Information
C-type lectin domain family 12 member B; macrophage antigen h
UniProt Protein Name
C-type lectin domain family 12 member B
Protein Family
UniProt Gene Name
CLEC12B
UniProt Synonym Gene Names
UNQ5782/PRO16089
UniProt Entry Name
CL12B_HUMAN
Uniprot Description
CLEC12B: Cell surface receptor that protects target cells against natural killer cell-mediated lysis. Modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.2
Cellular Component: plasma membrane; integral to membrane
Molecular Function: carbohydrate binding
Research Articles on CLEC12B
Similar Products
Product Notes
The Human CLEC12B clec12b (Catalog #AAA7217017) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7217017 ELISA Kit recognizes Human CLEC12B. It is sometimes possible for the material contained within the vial of "C type lectin domain family 12 member B (CLEC12B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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