Typical Testing Data/Standard Curve (for reference only)
Human bone sialoprotein, BSP ELISA Kit | BSP elisa kit
Human bone sialoprotein, BSP ELISA Kit
Gene Names
IBSP; BSP; BNSP; SP-II; BSP-II
Reactivity
Human
Synonyms
bone sialoprotein; BSP; Human bone sialoprotein; BSP ELISA Kit; BSP elisa kit
Reactivity
Human
Sequence Length
317
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.5ng/ml - 150ng/ml
Sensitivity
0.21ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BSP elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Bone Sialoprotein (also known as BSP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human BSP antibody. BSP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human BSP Antibody is added and binds to BSP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BSP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human BSP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human BSP antibody. BSP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human BSP Antibody is added and binds to BSP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BSP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human BSP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,148 Da
NCBI Official Full Name
bone sialoprotein
NCBI Official Synonym Full Names
integrin binding sialoprotein
NCBI Official Symbol
IBSP
NCBI Official Synonym Symbols
BSP; BNSP; SP-II; BSP-II
NCBI Protein Information
bone sialoprotein 2
UniProt Protein Name
Bone sialoprotein 2
Protein Family
UniProt Gene Name
IBSP
UniProt Synonym Gene Names
BNSP; BSP II
NCBI Description
The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]
Uniprot Description
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
Research Articles on BSP
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Product Notes
The Human BSP ibsp (Catalog #AAA163216) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA163216 ELISA Kit recognizes Human BSP. It is sometimes possible for the material contained within the vial of "bone sialoprotein, BSP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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