Mouse anti-Human TRUB1 Polyclonal Antibody | anti-Trub1 antibody

TRUB1 (Probable tRNA Pseudouridine Synthase 1, PUS4)

Cat. Num. AAA641945

• Gene Names: Trub1; C76870; AI448235; BB118943; 2610009I02Rik; 9030425C13Rik
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TRUB1; Polyclonal Antibody; TRUB1 (Probable tRNA Pseudouridine Synthase 1; PUS4); Anti -TRUB1 (Probable tRNA Pseudouridine Synthase 1; anti-Trub1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRUB1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MAASEAAVVSSPSLKTDTSPVLETAGTVAAMAATPSARAAAAVVAAAARTGSEARVSKAALATKLLSLSGVFAVHKPKGPTSAELLNRLKEKLLAEAGMPSPEWTKRKKQTLKIGHGGTLDSAARGVLVVGIGSGTKMLTSMLSGSKRYTAIGELGKATDTLDSTGRVTEEKPYDKITQEDIEGILQKFTGNIMQVPPLYSALKKDGQRLSTLMKRGEVVEAKPARPVTVYSISLQKFQPPFFTLDVECGGGFYIRSLVSDIGKELSSCANVLELTRTKQGPFTLEEHALPEDKWTIDDIAQSLEHCSSLFPAELALKKSKPESNEQVLSCEYITLNEPKREDDVIKTC

• Applicable Applications for anti-Trub1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human TRUB1, aa1-349 (NP_631908.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TRUB1 expression in transfected 293T cell line by TRUB1 polyclonal antibody. Lane 1: TRUB1 transfected lysate (38.39kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to TRUB1 on HeLa cell. [antibody concentration 10ug/ml].)

Product Categories/Family for anti-Trub1 antibody

Antibodies; Abs to Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 32449933
• NCBI GeneID: 72133
• UniProt Accession #: Q8C0D0; Q5EBH7; Q9D331
• Molecular Weight: 36,348 Da
• NCBI Official Full Name: Trub1 protein, partial
• NCBI Official Synonym Full Names: TruB pseudouridine (psi) synthase homolog 1 (E. coli)
• NCBI Official Symbol: Trub1
• NCBI Official Synonym Symbols: C76870; AI448235; BB118943; 2610009I02Rik; 9030425C13Rik
• NCBI Protein Information: probable tRNA pseudouridine synthase 1
• UniProt Protein Name: Probable tRNA pseudouridine synthase 1
• Protein Family: Probable tRNA pseudouridine synthase
• UniProt Gene Name: Trub1
• UniProt Entry Name: TRUB1_MOUSE

Uniprot Description

TRUB1: May be responsible for synthesis of pseudouridine from uracil in transfer RNAs. Belongs to the pseudouridine synthase TruB family.

Protein type: Isomerase; RNA processing; EC 5.4.99.-

Molecular Function: beta-amyrin synthase activity; RNA binding; pseudouridine synthase activity; lupeol synthase activity; isomerase activity

Biological Process: RNA processing; pseudouridine synthesis; tRNA processing; RNA modification