Human Bone Morphogenetic Protein 15 (BMP15) ELISA Kit | BMP15 elisa kit

Human Bone Morphogenetic Protein 15 (BMP15) ELISA Kit

Cat. Num. AAA451365

• Gene Names: BMP15; ODG2; POF4; GDF9B
• Reactivity: Human
• Synonyms: Bone Morphogenetic Protein 15 (BMP15); Human Bone Morphogenetic Protein 15 (BMP15) ELISA Kit; GDF9B; GDF9-B; ODG2; POF4; Growth/differentiation factor 9B; BMP15 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BMP15. No significant cross-reactivity or interference between BMP15 and analogues was observed.
• Sequence Length: 392

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BMP15 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BMP15 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP15 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BMP15 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BMP15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMP15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BMP15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 257743454
• NCBI GeneID: 9210
• NCBI Accession #: NP_005439.2
• NCBI GenBank Nucleotide #: NM_005448.2
• UniProt Accession #: O95972; Q17RM6; Q5JST1; Q9UMS1
• Molecular Weight: 45,055 Da
• NCBI Official Full Name: bone morphogenetic protein 15 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 15
• NCBI Official Symbol: BMP15
• NCBI Official Synonym Symbols: ODG2; POF4; GDF9B
• NCBI Protein Information: bone morphogenetic protein 15
• UniProt Protein Name: Bone morphogenetic protein 15
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP15
• UniProt Synonym Gene Names: GDF9B; BMP-15; GDF-9B

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate subunits of a disulfide-linked homodimer, or alternatively, a heterodimer, with the related protein, growth differentiation factor 9 (GDF9). This protein plays a role in oocyte maturation and follicular development, through activation of granulosa cells. Defects in this gene are the cause of ovarian dysgenesis and are associated with premature ovarian failure. [provided by RefSeq, Aug 2016]

Uniprot Description

May be involved in follicular development. Oocyte-specific growth/differentiation factor that stimulates folliculogenesis and granulosa cell (GC) growth.