Rabbit anti-Human FZD1 Polyclonal Antibody | anti-FZD1 antibody

FZD1 Antibody

Cat. Num. AAA7053361

• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: FZD1; Polyclonal Antibody; FZD1 Antibody; Frizzled-1; Fz-1; hFz1; FzE1; anti-FZD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 647

• Applicable Applications for anti-FZD1 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human Frizzled-1 protein (73-322AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7053361 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7053361 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of MCF-7 cells using MBS7053361 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-FZD1 antibody

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B.

NCBI and Uniprot Product Information

• NCBI GI #: 4503825
• NCBI GeneID: 8321
• NCBI Accession #: NP_003496.1
• NCBI GenBank Nucleotide #: NM_003505.1
• UniProt Accession #: Q9UP38; O94815; Q549T8; A4D1E8
• Molecular Weight: 71,158 Da
• NCBI Official Full Name: frizzled-1
• NCBI Official Synonym Full Names: frizzled class receptor 1
• NCBI Official Symbol: FZD1
• NCBI Protein Information: frizzled-1
• UniProt Protein Name: Frizzled-1
• Protein Family: Frizzled
• UniProt Gene Name: FZD1
• UniProt Synonym Gene Names: Fz-1; hFz1

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD1: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Activated by Wnt3A, Wnt3, Wnt1 and to a lesser extent Wnt2, but not by Wnt4, Wnt5A, Wnt5B, Wnt6, Wnt7A or Wnt7B. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 7q21.13

Cellular Component: cell surface; focal adhesion; integral to membrane; plasma membrane

Molecular Function: frizzled binding; G-protein coupled receptor activity; PDZ domain binding; protein binding; receptor binding; Wnt receptor activity; Wnt-protein binding

Biological Process: cell-cell signaling; neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; response to drug; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, planar cell polarity pathway