Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) Standard Curve.)
Human Bone Morphogenetic Protein 15 (BMP-15/GDF-9B) ELISA Kit | BMP-15/GDF-9B elisa kit
Human Bone Morphogenetic Protein 15 (BMP-15/GDF-9B) ELISA Kit
Gene Names
BMP15; bmp-15
Reactivity
Human
Synonyms
Bone Morphogenetic Protein 15 (BMP-15/GDF-9B); Human Bone Morphogenetic Protein 15 (BMP-15/GDF-9B) ELISA Kit; BMP15; GDF9B; ODG2; POF4; growth/differentiation factor 9B; BMP-15/GDF-9B elisa kit
Reactivity
Human
Specificity
Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) ELISA Kit has high sensitivity and excellent specificity for detection of Human BMP15. No significant cross-reactivity or interference between Human BMP15 and analogues was observed.
Sequence Length
394
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) Standard Curve.)
Related Product Information for BMP-15/GDF-9B elisa kit
Description: This Human Bone morphogenetic protein 15 (BMP-15/GDF-9B) ELISA Kit employs a two-site sandwich ELISA to quantitate BMP15 in samples. An antibody specific for BMP15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Bone morphogenetic protein 15 is a member of the TGF-beta superfamily. It is a paracrine signaling molecule involved in oocyte and follicular development. Using Northern blot analysis, BMP15 has been shown to be exclusively expressed in the ovaries. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The transforming growth factor-beta superfamily includes large families of growth and differentiation factors. It is thought that this protein may be involved in oocyte maturation and follicular development as a homodimer or by forming heterodimers with a related protein, Gdf9. Defects in this gene are the cause of ovarian dysgenesis 2.
Background: Bone morphogenetic protein 15 is a member of the TGF-beta superfamily. It is a paracrine signaling molecule involved in oocyte and follicular development. Using Northern blot analysis, BMP15 has been shown to be exclusively expressed in the ovaries. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The transforming growth factor-beta superfamily includes large families of growth and differentiation factors. It is thought that this protein may be involved in oocyte maturation and follicular development as a homodimer or by forming heterodimers with a related protein, Gdf9. Defects in this gene are the cause of ovarian dysgenesis 2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
bone morphogenetic protein 15
NCBI Official Synonym Full Names
bone morphogenetic protein 15
NCBI Official Symbol
BMP15
NCBI Official Synonym Symbols
bmp-15
NCBI Protein Information
bone morphogenetic protein 15
UniProt Protein Name
Bone morphogenetic protein 15
UniProt Gene Name
BMP15
UniProt Synonym Gene Names
BMP-15
UniProt Entry Name
BMP15_BOVIN
Research Articles on BMP-15/GDF-9B
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Product Notes
The Human BMP-15/GDF-9B bmp15 (Catalog #AAA9716267) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716267 ELISA Kit recognizes Human BMP-15/GDF-9B. It is sometimes possible for the material contained within the vial of "Bone Morphogenetic Protein 15 (BMP-15/GDF-9B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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