Human BMPR2 ELISA Kit | BMPR2 elisa kit

Human BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit

Cat. Num. AAA2509005

• Gene Names: BMPR2; BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
• Reactivity: Human
• Synonyms: BMPR2; Human BMPR2 (Bone Morphogenetic Protein Receptor II) ELISA Kit; BMPR2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human BMPR2. No significant cross-reactivity or interference between Human BMPR2 and analogues was observed.
• Sequence Length: 1038

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human BMPR2 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMPR2 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BMPR2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BMPR2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BMPR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BMPR2. You can calculate the concentration of BMPR2 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15451916
• NCBI GeneID: 659
• NCBI Accession #: NP_001195.2
• NCBI GenBank Nucleotide #: NM_001204.6
• UniProt Accession #: Q13873; Q13161; Q16569; Q4ZG08; Q53SA5; Q585T8
• Molecular Weight: 59,963 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-2
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type II (serine/threonine kinase)
• NCBI Official Symbol: BMPR2
• NCBI Official Synonym Symbols: BMR2; PPH1; BMPR3; BRK-3; POVD1; T-ALK; BMPR-II
• NCBI Protein Information: bone morphogenetic protein receptor type-2; BMP type II receptor; BMP type-2 receptor; BMPR-2; bone morphogenetic protein receptor type II; type II activin receptor-like kinase; type II receptor for bone morphogenetic protein-4
• UniProt Protein Name: Bone morphogenetic protein receptor type-2
• Protein Family: Bone morphogenetic protein receptor
• UniProt Gene Name: BMPR2
• UniProt Synonym Gene Names: PPH1; BMP type-2 receptor; BMPR-2; BMP type II receptor; BMPR-II
• UniProt Entry Name: BMPR2_HUMAN

NCBI Description

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease. [provided by RefSeq, Jul 2008]

Uniprot Description

BMPR2: a serine/threonine-protein kinase receptor for bone morphogenetic protein (BMP). Binds to BMP-7, BMP-2 and, less efficiently, BMP-4. Binding is weak but enhanced by the presence of type I receptors for BMPs. On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Defects in BMPR2 are the cause of primary pulmonary hypertension (PPH1), a weakly penetrant dominant disorder, associated with lesions in pulmonary arterioles, elevated pulmonary arterial pressure, and right ventricular failure.

Protein type: EC 2.7.11.30; Protein kinase, TKL; Cell cycle regulation; Kinase, protein; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 2q33-q34

Cellular Component: extracellular space; cell surface; cell soma; integral to plasma membrane; dendrite; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; caveola

Molecular Function: transforming growth factor beta receptor activity; protein binding; metal ion binding; growth factor binding; activin receptor activity, type II; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: transcription from RNA polymerase II promoter; limb development; regulation of lung blood pressure; negative regulation of systemic arterial blood pressure; activin receptor signaling pathway; chondrocyte development; positive regulation of bone mineralization; cellular response to starvation; protein amino acid phosphorylation; negative regulation of vasoconstriction; regulation of cell proliferation; anterior/posterior pattern formation; BMP signaling pathway; positive regulation of osteoblast differentiation; mesoderm formation; proteoglycan biosynthetic process; transmembrane receptor protein serine/threonine kinase signaling pathway; lymphangiogenesis; positive regulation of axon extension involved in axon guidance; blood vessel remodeling; positive regulation of endothelial cell proliferation; brain development; negative regulation of cell growth; vascular endothelial growth factor receptor signaling pathway; alveolus development; positive regulation of BMP signaling pathway

Disease: Pulmonary Venoocclusive Disease 1, Autosomal Dominant; Pulmonary Hypertension, Primary, 1