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Western Blot (WB) (Western blot analysis of LAP3 expression in SW620 (A), HL60 (B), mouse liver (C), mouse heart (D) whole cell lysates.)

Rabbit LAP3 Polyclonal Antibody | anti-LAP3 antibody

Anti-LAP3 Antibody

Gene Names
LAP3; LAP; PEPS; LAPEP; HEL-S-106
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
LAP3; Polyclonal Antibody; Anti-LAP3 Antibody; LAPEP; PEPS; Cytosol aminopeptidase; Leucine aminopeptidase 3; LAP-3; Leucyl aminopeptidase; Peptidase S; Proline aminopeptidase; Prolyl aminopeptidase; anti-LAP3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of LAP3 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
488
Applicable Applications for anti-LAP3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1/500 - 1/2000; IHC: 1/50 - 1/200
Immunogen
Recombinant full length protein of human LAP3
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of LAP3 expression in SW620 (A), HL60 (B), mouse liver (C), mouse heart (D) whole cell lysates.)

Western Blot (WB) (Western blot analysis of LAP3 expression in SW620 (A), HL60 (B), mouse liver (C), mouse heart (D) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of LAP3 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of LAP3 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-LAP3 antibody
Rabbit polyclonal antibody to LAP3

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,771 Da
NCBI Official Full Name
cytosol aminopeptidase
NCBI Official Synonym Full Names
leucine aminopeptidase 3
NCBI Official Symbol
LAP3
NCBI Official Synonym Symbols
LAP; PEPS; LAPEP; HEL-S-106
NCBI Protein Information
cytosol aminopeptidase
UniProt Protein Name
Cytosol aminopeptidase
Protein Family
UniProt Gene Name
LAP3
UniProt Synonym Gene Names
LAPEP; PEPS; LAP-3
UniProt Entry Name
AMPL_HUMAN

Uniprot Description

LAP3: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Belongs to the peptidase M17 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Protease; Mitochondrial; Amino Acid Metabolism - arginine and proline; EC 3.4.11.1; EC 3.4.11.5; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: cytoplasm; focal adhesion; mitochondrion; nucleoplasm; nucleus; trans-Golgi network

Molecular Function: aminopeptidase activity; manganese ion binding; metalloexopeptidase activity

Biological Process: proteolysis

Research Articles on LAP3

Similar Products

Product Notes

The LAP3 lap3 (Catalog #AAA8245672) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LAP3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LAP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200. Researchers should empirically determine the suitability of the LAP3 lap3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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