Human Beta-defensin 2 (DEFB2) ELISA Kit | DEFB2 elisa kit

Human Beta-defensin 2 (DEFB2) ELISA Kit

Cat. Num. AAA2801782

• Gene Names: DEFB4B; DEFB4P
• Reactivity: Human
• Synonyms: Beta-defensin 2 (DEFB2); Human Beta-defensin 2 (DEFB2) ELISA Kit; DEFB-2; DEFB102; DEFB2; HBD-2; SAP1; defensin; beta 2; skin-antimicrobial peptide 1; DEFB2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human DEFB2. No significant cross-reactivity or interference between Human DEFB2 and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate DEFB2 in samples. An antibody specific for DEFB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDEFB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 3023383
• NCBI GeneID: 100289462
• UniProt Accession #: O15263; Q52LC0
• Molecular Weight: 64
• NCBI Official Full Name: Beta-defensin 4A
• NCBI Official Synonym Full Names: defensin, beta 4B
• NCBI Official Symbol: DEFB4B
• NCBI Official Synonym Symbols: DEFB4P
• NCBI Protein Information: beta-defensin 4B; defensin, beta 4, pseudogene
• UniProt Protein Name: Beta-defensin 4A
• UniProt Gene Name: DEFB4A
• UniProt Synonym Gene Names: DEFB102; DEFB2; DEFB4; BD-2; hBD-2; SAP1
• UniProt Entry Name: DFB4A_HUMAN

NCBI Description

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Oct 2014]

Uniprot Description

defensin, beta 2: Has antibacterial activity (Potential). Belongs to the beta-defensin family. LAP/TAP subfamily.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: Golgi lumen; extracellular region

Biological Process: G-protein coupled receptor protein signaling pathway; defense response to bacterium; innate immune response; immune response; chemotaxis