Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-defensin 114 (DEFB114) Standard Curve.)
Human Beta-Defensin 114 (DEFB114) ELISA Kit | DEFB114 elisa kit
Human Beta-Defensin 114 (DEFB114) ELISA Kit
Gene Names
DEFB114; DEFB14; DEFB-14
Reactivity
Human
Synonyms
Beta-Defensin 114 (DEFB114); Human Beta-Defensin 114 (DEFB114) ELISA Kit; DEFB114; DEFB-14; beta-defensin 114; defensin; beta 14; DEFB114 elisa kit
Reactivity
Human
Specificity
Human Beta-defensin 114 (DEFB114) ELISA Kit has high sensitivity and excellent specificity for detection of Human DEFB114. No significant cross-reactivity or interference between Human DEFB114 and analogues was observed.
Sequence Length
39
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-defensin 114 (DEFB114) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-defensin 114 (DEFB114) Standard Curve.)
Related Product Information for DEFB114 elisa kit
Description: This Human Beta-defensin 114 (DEFB114) ELISA Kit employs a two-site sandwich ELISA to quantitate DEFB114 in samples. An antibody specific for DEFB114 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFB114 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB114 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB114 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. The protein encoded by DEFB114 is a beta-defensin with antimicrobial activity against E. coli, S. aureus, and C. albicans. The encoded protein also binds and neutralizes lipopolysaccharide (LPS), a factor involved in inflammatory diseases and male reproductive issues.
Background: Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. The protein encoded by DEFB114 is a beta-defensin with antimicrobial activity against E. coli, S. aureus, and C. albicans. The encoded protein also binds and neutralizes lipopolysaccharide (LPS), a factor involved in inflammatory diseases and male reproductive issues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
defensin beta 114, partial
NCBI Official Synonym Full Names
defensin beta 114
NCBI Official Symbol
DEFB114
NCBI Official Synonym Symbols
DEFB14; DEFB-14
NCBI Protein Information
beta-defensin 114
UniProt Protein Name
Beta-defensin 114
Protein Family
UniProt Gene Name
DEFB114
UniProt Synonym Gene Names
DEFB14; DEFB-14
NCBI Description
Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. The protein encoded by this gene is a beta-defensin with antimicrobial activity against E. coli, S. aureus, and C. albicans. The encoded protein also binds and neutralizes lipopolysaccharide (LPS), a factor involved in inflammatory diseases and male reproductive issues. [provided by RefSeq, Nov 2014]
Uniprot Description
Has a salt-sensitive antimicrobial activity against Gram-negative bacteria, including E.coli, Gram-positive, including S.aureus, and fungi, including C.albicans. Binds to and neutralizes bacterial lipopolysaccharides (LPS), abolishing TNF production by macrophages challenged with LPS. Rescues the LPS-induced reduction of sperm motility in vitro and may protect from LPS-induced lethality.
Research Articles on DEFB114
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Product Notes
The Human DEFB114 defb114 (Catalog #AAA9715781) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715781 ELISA Kit recognizes Human DEFB114. It is sometimes possible for the material contained within the vial of "Beta-Defensin 114 (DEFB114), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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