Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-defensin 130 (DEFB130) Standard Curve.)
Human Beta-Defensin 130 (DEFB130) ELISA Kit | DEFB130 elisa kit
Human Beta-Defensin 130 (DEFB130) ELISA Kit
Gene Names
DEFB130A; DEFB30; DEFB-30; DEFB130; DEFB130L
Reactivity
Human
Synonyms
Beta-Defensin 130 (DEFB130); Human Beta-Defensin 130 (DEFB130) ELISA Kit; DEFB130; DEFB-30; beta-defensin 130; defensin; beta 30; DEFB130 elisa kit
Reactivity
Human
Specificity
Human Beta-defensin 130 (DEFB130) ELISA Kit has high sensitivity and excellent specificity for detection of Human DEFB130. No significant cross-reactivity or interference between Human DEFB130 and analogues was observed.
Sequence Length
79
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-defensin 130 (DEFB130) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Beta-defensin 130 (DEFB130) Standard Curve.)
Related Product Information for DEFB130 elisa kit
Description: This Human Beta-defensin 130 (DEFB130) ELISA Kit employs a two-site sandwich ELISA to quantitate DEFB130 in samples. An antibody specific for DEFB130 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFB130 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB130 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB130 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by DEFB130A is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with DEFB130A mapping to a cluster at 8p23.
Background: Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by DEFB130A is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with DEFB130A mapping to a cluster at 8p23.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
beta-defensin 130A
NCBI Official Synonym Full Names
defensin beta 130A
NCBI Official Symbol
DEFB130A
NCBI Official Synonym Symbols
DEFB30; DEFB-30; DEFB130; DEFB130L
NCBI Protein Information
beta-defensin 130A
UniProt Protein Name
Beta-defensin 130
Protein Family
UniProt Gene Name
DEFB130
UniProt Synonym Gene Names
DEFB30
DEFB130L; DEFB-30
DEFB130L; DEFB-30
UniProt Entry Name
DB130_HUMAN
NCBI Description
Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 8p23. [provided by RefSeq, Nov 2014]
Uniprot Description
defensin, beta 30: Has antibacterial activity (Potential). Belongs to the beta-defensin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: extracellular region
Biological Process: defense response to bacterium
Research Articles on DEFB130
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Product Notes
The Human DEFB130 defb130 (Catalog #AAA9715772) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715772 ELISA Kit recognizes Human DEFB130. It is sometimes possible for the material contained within the vial of "Beta-Defensin 130 (DEFB130), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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