Typical Testing Data/Standard Curve (for reference only)
Human beta adrenergic receptor kinase 1 (ADRBK1) ELISA Kit | ADRBK1 elisa kit
Human beta adrenergic receptor kinase 1 (ADRBK1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ADRBK1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADRBK1 antibody and an ADRBK1-HRP conjugate. The assay sample and buffer are incubated together with ADRBK1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRBK1 concentration since ADRBK1 from samples and ADRBK1-HRP conjugate compete for the anti-ADRBK1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRBK1 from the sample, fewer sites are left to bind ADRBK1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRBK1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]
Uniprot Description
GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.
Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.15; Protein kinase, Ser/Thr (non-receptor); AGC group; GRK family; BARK subfamily
Chromosomal Location of Human Ortholog: 11q13.1
Cellular Component: membrane; cytoplasm; plasma membrane; cytosol
Molecular Function: G-protein coupled receptor kinase activity; protein binding; Edg-2 lysophosphatidic acid receptor binding; beta-adrenergic receptor kinase activity; alpha-2A adrenergic receptor binding; ATP binding; protein kinase activity
Biological Process: epidermal growth factor receptor signaling pathway; desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; acetylcholine receptor signaling, muscarinic pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; peptidyl-threonine phosphorylation; signal transduction; negative regulation of striated muscle contraction; peptidyl-serine phosphorylation; negative regulation of the force of heart contraction by chemical signal; phospholipase C activation; receptor internalization; innate immune response; positive regulation of catecholamine secretion; cardiac muscle contraction
Research Articles on ADRBK1
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Product Notes
The Human ADRBK1 adrbk1 (Catalog #AAA7209250) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7209250 ELISA Kit recognizes Human ADRBK1. It is sometimes possible for the material contained within the vial of "beta adrenergic receptor kinase 1 (ADRBK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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