Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Milk fat globule-EGF factor 8 protein (MFGE8) Standard Curve.)
Rat Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA Kit | MFGE8 elisa kit
Rat Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA Kit
Gene Names
Mfge8; AGS; MFGME8; OAcGD3S; MFGMP-E8
Reactivity
Rat
Synonyms
Milk Fat Globule-EGF Factor 8 Protein (MFGE8); Rat Milk Fat Globule-EGF Factor 8 Protein (MFGE8) ELISA Kit; MFGE8; BA46; EDIL1; HsT19888; MFG-E8; OAcGD3S; SED1; SPAG10; hP47; O-acetyl disialoganglioside synthase; lactadherin; medin; sperm associated antigen 10; sperm surface protein hP47; MFGE8 elisa kit
Reactivity
Rat
Specificity
Rat Milk fat globule-EGF factor 8 protein (MFGE8) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MFGE8. No significant cross-reactivity or interference between Rat MFGE8 and analogues was observed.
Sequence Length
483
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Milk fat globule-EGF factor 8 protein (MFGE8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Milk fat globule-EGF factor 8 protein (MFGE8) Standard Curve.)
Related Product Information for MFGE8 elisa kit
Description: This Rat Milk fat globule-EGF factor 8 protein (MFGE8) ELISA Kit employs a two-site sandwich ELISA to quantitate MFGE8 in samples. An antibody specific for MFGE8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFGE8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFGE8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFGE8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MFGE8 encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants.
Background: MFGE8 encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
lactadherin isoform 1
NCBI Official Synonym Full Names
milk fat globule-EGF factor 8 protein
NCBI Official Symbol
Mfge8
NCBI Official Synonym Symbols
AGS; MFGME8; OAcGD3S; MFGMP-E8
NCBI Protein Information
lactadherin
UniProt Protein Name
Lactadherin
Protein Family
UniProt Gene Name
Mfge8
UniProt Synonym Gene Names
Ags; MFG-E8; AGS
UniProt Entry Name
MFGM_RAT
NCBI Description
involved in the O-acetylation of GD3 ganglioside sialic acid [RGD, Feb 2006]
Uniprot Description
Function: Contributes to phagocytic removal of apoptotic cells in many tissues. Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Appears to participate in the O-acetylation of GD3 ganglioside sialic acid.
Research Articles on MFGE8
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Product Notes
The Rat MFGE8 mfge8 (Catalog #AAA9712284) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712284 ELISA Kit recognizes Rat MFGE8. It is sometimes possible for the material contained within the vial of "Milk Fat Globule-EGF Factor 8 Protein (MFGE8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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