Human Baculoviral IAP Repeat Containing Protein 7 (BIRC7) ELISA Kit | BIRC7 elisa kit

Human Baculoviral IAP Repeat Containing Protein 7 (BIRC7) ELISA Kit

Cat. Num. AAA456321

• Gene Names: BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• Reactivity: Human
• Synonyms: Baculoviral IAP Repeat Containing Protein 7 (BIRC7); Human Baculoviral IAP Repeat Containing Protein 7 (BIRC7) ELISA Kit; BIRC7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BIRC7. No significant cross-reactivity or interference between BIRC7 and analogues was observed.
• Sequence Length: 280

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.073ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BIRC7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BIRC7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BIRC7 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BIRC7 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BIRC7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BIRC7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BIRC7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BIRC7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11545910
• NCBI GeneID: 79444
• NCBI Accession #: NP_071444.1
• NCBI GenBank Nucleotide #: NM_022161.3
• UniProt Accession #: Q96CA5; Q9BQV0; Q9H2A8; Q9HAP7
• Molecular Weight: 30,866 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 7 isoform beta
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 7
• NCBI Official Symbol: BIRC7
• NCBI Official Synonym Symbols: KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 7
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 7
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC7
• UniProt Synonym Gene Names: KIAP; LIVIN; MLIAP; RNF50; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin

NCBI Description

This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]

Uniprot Description

Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing.