Human B7.H3 gene ELISA Kit | B7.H3 elisa kit

Human B7.H3 gene ELISA Kit

Cat. Num. AAA727830

• Gene Names: CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
• Reactivity: Human
• Synonyms: B7.H3 gene; Human B7.H3 gene ELISA Kit; B7.H3 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This B7.H3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human B7.H3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for B7.H3 elisa kit

Principle of the Assay: B7.H3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for B7.H3. Standards or samples are then added to the microtiter plate wells and B7.H3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of B7.H3 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for B7.H3 are added to each well to "sandwich" the B7.H3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain B7.H3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B7.H3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for B7.H3 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 52694705
• NCBI GeneID: 80381
• UniProt Accession #: Q5ZPR3; Q6P5Y4; Q6UXI2; Q8NBI8; Q8NC34; Q8NCB6; Q9BXR1
• Molecular Weight: 57,165 Da
• NCBI Official Full Name: B7-H3 protein
• NCBI Official Synonym Full Names: CD276 molecule
• NCBI Official Symbol: CD276
• NCBI Official Synonym Symbols: B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
• NCBI Protein Information: CD276 antigen; B7 homolog 3; costimulatory molecule
• UniProt Protein Name: CD276 antigen
• UniProt Gene Name: CD276
• UniProt Synonym Gene Names: B7H3; PSEC0249; UNQ309/PRO352; B7-H3
• UniProt Entry Name: CD276_HUMAN

NCBI Description

The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: cell proliferation; positive regulation of osteoblast differentiation; regulation of immune response; negative regulation of T cell proliferation; T cell activation; negative regulation of inflammatory response; negative regulation of interleukin-2 biosynthetic process; immune response; positive regulation of T cell proliferation; positive regulation of bone mineralization; positive regulation of interferon-gamma biosynthetic process; negative regulation of interferon-gamma biosynthetic process