Western Blot (WB)
Rabbit SHC Polyclonal Antibody | anti-SHC antibody
SHC Antibody
Reactivity
Mouse, RatPredicted: Human
Applications
ELISA, Immunoprecipitation, Western Blot
Synonyms
SHC; Polyclonal Antibody; SHC Antibody; p46; p52; p66; SH2 domain protein C1; Shc; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; SHC1; SHCA; Src homology 2 domain containing transforming protein C1 antibody; anti-SHC antibody
Host
Rabbit
Reactivity
Mouse, Rat
Predicted: Human
Predicted: Human
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
0.55-0.68 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
474
Applicable Applications for anti-SHC antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Dot Blot: 1:10,000
ELISA: 1:10,000
Western Blot: 1:500
ELISA: 1:10,000
Western Blot: 1:500
Immunogen
Synthetic peptide taken within amino acid region 425-485 on mouse ShcA protein.
Expression
Widely expressed. Expressed in neural stem cells but absent in mature neurons.
Molecular Function
Angiogenesis, Growth regulation
Structure
Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. Once activated, binds to GRB2. Interacts with tyrosine-phosphorylated CD3T and DDR2. Interacts with the N-terminal region of APS. Interacts with phosphorylated LRP1 and IRS4. Interacts with INPP5D/SHIP1 and INPPL1/SHIP2. Interacts with TRIM31. Interacts with PTPN6/SHP (tyrosine phosphorylated). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTT (By similarity). Interacts with ALK, GAB2, GRB7 and KIT. Interacts with FLT4 (tyrosine-phosphorylated). Interacts with EPHB1 and GRB2; activates the MAPK/ERK cascade to regulate cell migration. Interacts with PDGFRB (tyrosine-phosphorylated). Interacts with ERBB4. Interacts with TEK/TIE2 (tyrosine-phosphorylated). Interacts with the Trk receptors NTRK1, NTRK2 and NTRK3; in a phosphotyrosine-dependent manner. Interacts with PTK2/FAK1.
Subcellular Location
Cytoplasm
Preparation and Storage
-20 degree C for long term storage
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-SHC antibody
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
Product Categories/Family for anti-SHC antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The SHC (Catalog #AAA540535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHC Antibody reacts with Mouse, Rat Predicted: Human and may cross-react with other species as described in the data sheet. AAA Biotech's SHC can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Dot Blot: 1:10,000 ELISA: 1:10,000 Western Blot: 1:500. Researchers should empirically determine the suitability of the SHC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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