Human B cell lymphoma/leukemia 11A (BCL11A) ELISA Kit | BCL11A elisa kit

Human B cell lymphoma/leukemia 11A (BCL11A) ELISA Kit

Cat. Num. AAA7224832

• Gene Names: BCL11A; EVI9; CTIP1; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL
• Reactivity: Human
• Synonyms: B cell lymphoma/leukemia 11A (BCL11A); Human B cell lymphoma/leukemia 11A (BCL11A) ELISA Kit; BCL11A elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This BCL11A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human BCL11A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BCL11A elisa kit

Principle of the assay: BCL11A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BCL11A antibody and an BCL11A-HRP conjugate. The assay sample and buffer are incubated together with BCL11A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL11A concentration since BCL11A from samples and BCL11A-HRP conjugate compete for the anti-BCL11A antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL11A from the sample, fewer sites are left to bind BCL11A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL11A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for BCL11A elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 20336305
• NCBI GeneID: 53335
• NCBI Accession #: NP_075044.2
• NCBI GenBank Nucleotide #: NM_022893.3
• Molecular Weight: 87,554 Da
• NCBI Official Full Name: B-cell lymphoma/leukemia 11A isoform 1
• NCBI Official Synonym Full Names: B-cell CLL/lymphoma 11A (zinc finger protein)
• NCBI Official Symbol: BCL11A
• NCBI Official Synonym Symbols: EVI9; CTIP1; ZNF856; HBFQTL5; BCL11A-L; BCL11A-S; BCL11a-M; BCL11A-XL
• NCBI Protein Information: B-cell lymphoma/leukemia 11A; EVI-9; BCL-11A; zinc finger protein 856; C2H2-type zinc finger protein; COUP-TF-interacting protein 1; ecotropic viral integration site 9 homolog; ecotropic viral integration site 9 protein homolog; B-cell CLL/lymphoma 11A (zinc finger protein) isoform 2; BCL11A B-cell CLL/lymphoma 11A (zinc finger protein) isoform 1
• UniProt Protein Name: B-cell lymphoma/leukemia 11A
• Protein Family: B-cell lymphoma/leukemia
• UniProt Gene Name: BCL11A
• UniProt Synonym Gene Names: CTIP1; EVI9; KIAA1809; ZNF856; BCL-11A; EVI-9
• UniProt Entry Name: BC11A_HUMAN

NCBI Description

This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Bcl-11A: Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; C2H2-type zinc finger protein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2p16.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein homodimerization activity; protein heterodimerization activity; metal ion binding; transcription corepressor activity

Biological Process: protein sumoylation; transcription, DNA-dependent; B cell differentiation; regulation of dendrite development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of axon extension; negative regulation of transcription from RNA polymerase II promoter; negative regulation of collateral sprouting; positive regulation of collateral sprouting; negative regulation of protein homooligomerization; T cell differentiation