Human Autophagy protein 5, ATG5 ELISA Kit | ATG5 elisa kit

Human Autophagy protein 5, ATG5 ELISA Kit

Cat. Num. AAA2602759

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
• Reactivity: Human
• Synonyms: Autophagy protein 5; ATG5; Human Autophagy protein 5; ATG5 ELISA Kit; ATG5 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 50 ng/ml-0.78 ng/ml
• Sensitivity: Up to 0.1 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATG5 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ATG5 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 554790362
• NCBI GeneID: 9474
• NCBI Accession #: NP_001273035.1
• NCBI GenBank Nucleotide #: NM_001286106.1
• UniProt Accession #: Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
• Molecular Weight: 32,447 Da
• NCBI Official Full Name: autophagy protein 5 isoform a
• NCBI Official Synonym Full Names: autophagy related 5
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; APG5-LIKE
• NCBI Protein Information: autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog
• UniProt Protein Name: Autophagy protein 5
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP
• UniProt Entry Name: ATG5_HUMAN

Uniprot Description

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; autophagy; innate immune response; negative regulation of protein ubiquitination; blood vessel remodeling; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation