Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using CEP135 antibody.)
Rabbit anti-Human, Mouse CEP135 Polyclonal Antibody | anti-CEP135 antibody
CEP135 Antibody
Gene Names
CEP135; CEP4; MCPH8; KIAA0635
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CEP135; Polyclonal Antibody; CEP135 Antibody; centrosomal protein 135 kDa; centrosomal protein 135kDa; Centrosomal protein 4; Centrosomal protein of 135 kDa; centrosome protein 4; centrosome protein cep135; Cep135; CEP4; CP135_HUMAN; anti-CEP135 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CEP135 antibody detects endogenous levels of total CEP135
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1140
Applicable Applications for anti-CEP135 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm, Cytoskeleton, Microtubule Organizing Center, Centrosome, Centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
Predicted Cross Reactivity
Pig, Horse, Sheep, Rabbit, Dog
Similarity
Pig (91%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using CEP135 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from COLO cells, using CEP135 antibody.)
Immunofluorescene (IF)
(MBS9605372 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9605372 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-CEP135 antibody
Function: Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
Subunit Structure: Interacts with DCTN2 (By similarity). Interacts with CEP250 (PubMed:18851962).
Similarity: Belongs to the CEP135/TSGA10 family.
Subunit Structure: Interacts with DCTN2 (By similarity). Interacts with CEP250 (PubMed:18851962).
Similarity: Belongs to the CEP135/TSGA10 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 134 kD
Predicted: 134 kDa
Predicted: 134 kDa
NCBI Official Full Name
centrosomal protein of 135 kDa
NCBI Official Synonym Full Names
centrosomal protein 135
NCBI Official Symbol
CEP135
NCBI Official Synonym Symbols
CEP4; MCPH8; KIAA0635
NCBI Protein Information
centrosomal protein of 135 kDa
UniProt Protein Name
Centrosomal protein of 135 kDa
Protein Family
UniProt Gene Name
CEP135
UniProt Synonym Gene Names
CEP4; KIAA0635; Cep135
NCBI Description
This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8. [provided by RefSeq, Jun 2012]
Uniprot Description
Involved in early centriole assembly/duplication/biogenesis/formation/. Required for centriole elongation. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner.
Research Articles on CEP135
Similar Products
Product Notes
The CEP135 cep135 (Catalog #AAA9605372) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CEP135 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CEP135 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the CEP135 cep135 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEP135, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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