Rabbit anti-Human FBXO31 Polyclonal Antibody | anti-FBXO31 antibody

FBXO31, ID (FBXO31, FBX14, FBX31, F-box only protein 31)

Cat. Num. AAA647397

• Gene Names: FBXO31; FBX14; Fbx31; FBXO14; pp2386
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: FBXO31; Polyclonal Antibody; ID (FBXO31; FBX14; FBX31; F-box only protein 31); Anti -FBXO31; anti-FBXO31 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-FBXO31 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: FBXO31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-338 amino acids from the Central region of human FBXO31.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FBXO31 Antibody (Center) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the FBXO31 antibody detected the FBXO31 protein (arrow).)

Related Product Information for anti-FBXO31 antibody

Members of the F-box protein family, such as FBXO31, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].

Product Categories/Family for anti-FBXO31 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 544186107
• NCBI GeneID: 79791
• NCBI Accession #: NP_001269612.1
• NCBI GenBank Nucleotide #: NM_001282683.1
• UniProt Accession #: Q5XUX0; Q5K680; Q8WYV1; Q96D73; Q9UFV4
• Molecular Weight: 60,664 Da
• NCBI Official Full Name: F-box only protein 31 isoform 2
• NCBI Official Synonym Full Names: F-box protein 31
• NCBI Official Symbol: FBXO31
• NCBI Official Synonym Symbols: FBX14; Fbx31; FBXO14; pp2386
• NCBI Protein Information: F-box only protein 31; SCF ubiquitin ligase specificity factor; putative breast cancer tumor-suppressor
• UniProt Protein Name: F-box only protein 31
• Protein Family: F-box only protein
• UniProt Gene Name: FBXO31
• UniProt Synonym Gene Names: FBX14; FBX31
• UniProt Entry Name: FBX31_HUMAN

NCBI Description

This gene is a member of the F-box family. Members are classified into three classes according to the substrate interaction domain, FBW for WD40 repeats, FBL for leucing-rich repeats, and FBXO for other domains. This protein, classified into the last category because of the lack of a recognizable substrate binding domain, has been proposed to be a component of the SCF ubiquitination complex. It is thought to bind and recruit substrate for ubiquitination and degradation. This protein may have a role in regulating the cell cycle as well as dendrite growth and neuronal migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

Function: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor. Ref.7 Ref.8

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Part of a SCF (SKP1-cullin-F-box) protein ligase complex. Ref.7 Ref.8

Tissue specificity: Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow. Ref.7

Developmental stage: Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level). Ref.7

Induction: By DNA damage. Increases after UV irradiation, X-ray irradiation, oxidative stress (H2O2) or addition of the chemotherapeutic DNA-damaging agents etoposide, adriamycin, cisplatin or fluorouracil. Ref.8

Post-translational modification: Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization.

Sequence similarities: Belongs to the FBXO31 family.Contains 1 F-box domain.

Sequence caution: The sequence AAH12748.1 differs from that shown. Reason: Erroneous initiation. The sequence AAL55855.1 differs from that shown. Reason: Frameshift at position 496. The sequence CAB55929.2 differs from that shown. Reason: Erroneous initiation.