Rat calmodulin 1 (phosphorylase kinase, delta) ELISA Kit | CALM1 elisa kit

Rat calmodulin, CAM ELISA Kit

Cat. Num. AAA703917

• Gene Names: Calm3; RNCAMIII
• Reactivity: Rat
• Synonyms: calmodulin 1 (phosphorylase kinase; delta); Rat calmodulin; CAM ELISA Kit; CAMI; DD132; PHKD; calmodulin 1; phosphorylase kinase; delta subunit; CALM1 CALM CAM CAM1; CALM2 CAM2 CAMB; CALM3 CALML2 CAM3 CAMC CAMIII; CALM1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat CAM. No significant cross-reactivity or interference between rat CAM and analogues was observed.
• Sequence Length: 149

• Samples: Serum, plasma
• Assay Type: Sandwich
• Detection Range: 15.6 ng/ml -1000 ng/ml.
• Sensitivity: The minimum detectable dose of rat CAM is typically less than 3.9 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CALM1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAM bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6978591
• NCBI GeneID: 24244
• NCBI Accession #: NP_036650.1
• NCBI GenBank Nucleotide #: NM_012518.3
• UniProt Accession #: P62161; P02593; P70667; P99014; Q61379; Q61380
• Molecular Weight: 16,838 Da
• NCBI Official Full Name: calmodulin
• NCBI Official Synonym Full Names: calmodulin 3
• NCBI Official Symbol: Calm3
• NCBI Official Synonym Symbols: RNCAMIII
• NCBI Protein Information: calmodulin; caM; Calmodulin III
• UniProt Protein Name: Calmodulin
• Protein Family: Calmodulin
• UniProt Gene Name: Calm1
• UniProt Synonym Gene Names: Calm; Cam; Cam1; Calm2; Cam2; Camb; Calm3; Cam3; Camc; CaM
• UniProt Entry Name: CALM_RAT

NCBI Description

putative calmodulin protein expressed predominantly in skeletal muscle and brain [RGD, Feb 2006]

Uniprot Description

Function: Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis