Human ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) ELISA Kit | ATP1a2 elisa kit

Human ATPase, Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) ELISA Kit

Cat. Num. AAA456836

• Gene Names: ATP1A2; FHM2; MHP2
• Reactivity: Human
• Synonyms: ATPase; Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2); Human ATPase; Na+/K+ Transporting Alpha 2 Polypeptide (ATP1a2) ELISA Kit; FHM2; MHP2; Migraine; Hemiplegic 2; Sodium pump subunit alpha-2; Sodium/potassium-transporting ATPase subunit alpha-2; ATP1a2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP1a2. No significant cross-reactivity or interference between ATP1a2 and analogues was observed.
• Sequence Length: 1020

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: <0.105ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP1a2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP1a2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP1a2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ATP1a2 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP1a2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATP1a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP1a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP1a2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502271
• NCBI GeneID: 477
• NCBI Accession #: NP_000693.1
• NCBI GenBank Nucleotide #: NM_000702.3
• UniProt Accession #: P50993; Q07059; Q5JW74; Q86UZ5; Q9UQ25; D3DVE4
• Molecular Weight: 112,265 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-2
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting subunit alpha 2
• NCBI Official Symbol: ATP1A2
• NCBI Official Synonym Symbols: FHM2; MHP2
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-2
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-2
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A2
• UniProt Synonym Gene Names: KIAA0778; Na(+)/K(+) ATPase alpha-2 subunit

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 2 subunit. Mutations in this gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. [provided by RefSeq, Oct 2008]

Uniprot Description

ATP1A2: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients. Defects in ATP1A2 are the cause of familial hemiplegic migraine type 2 (FHM2). FHM2 is a rare, severe, autosomal dominant subtype of migraine characterized by aura and some hemiparesis. Defects in ATP1A2 are a cause of alternating hemiplegia of childhood 1 (AHC1). AHC is typically distinguished from familial hemiplegic migraine by infantile onset of the symptoms and high prevalence of associated neurological deficits that become increasingly obvious with age. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

Protein type: EC 3.6.3.9; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: caveola; cytoplasm; dendritic spine; endosome; membrane; myelin sheath; plasma membrane; sodium:potassium-exchanging ATPase complex; T-tubule

Molecular Function: ATP binding; ATPase activity; chaperone binding; drug binding; potassium ion binding; protein binding; sodium ion binding; sodium:potassium-exchanging ATPase activity

Biological Process: adult locomotory behavior; ATP hydrolysis coupled proton transport; ATP metabolic process; cardiac muscle contraction; cellular potassium ion homeostasis; cellular sodium ion homeostasis; establishment or maintenance of transmembrane electrochemical gradient; locomotion; negative regulation of heart contraction; negative regulation of striated muscle contraction; neurotransmitter uptake; potassium ion import; potassium ion transport; reduction of cytosolic calcium ion concentration; regulation of blood pressure; regulation of glutamate uptake involved in transmission of nerve impulse; regulation of respiratory gaseous exchange by neurological system process; regulation of smooth muscle contraction; regulation of striated muscle contraction; regulation of synaptic transmission, glutamatergic; regulation of the force of heart contraction; regulation of vasoconstriction; response to nicotine; sodium ion transport; visual learning

Disease: Alternating Hemiplegia Of Childhood 1; Migraine, Familial Hemiplegic, 2