Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit | ATP4a elisa kit

Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

Cat. Num. AAA456025

• Gene Names: ATP4A; ATP6A
• Reactivity: Human
• Synonyms: ATPase; H+/K+ Exchanging Alpha Polypeptide (ATP4a); Human ATPase; H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit; ATP4a elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP4a. No significant cross-reactivity or interference between ATP4a and analogues was observed.
• Sequence Length: 1,035

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP4a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP4a were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP4a elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATP4a in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP4a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATP4a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP4a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP4a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51944966
• NCBI GeneID: 495
• NCBI Accession #: NP_000695.2
• NCBI GenBank Nucleotide #: NM_000704.2
• UniProt Accession #: P20648; O00738
• Molecular Weight: 114,119 Da
• NCBI Official Full Name: potassium-transporting ATPase alpha chain 1
• NCBI Official Synonym Full Names: ATPase H+/K+ transporting alpha subunit
• NCBI Official Symbol: ATP4A
• NCBI Official Synonym Symbols: ATP6A
• NCBI Protein Information: potassium-transporting ATPase alpha chain 1
• UniProt Protein Name: Potassium-transporting ATPase alpha chain 1
• Protein Family: Potassium-transporting ATPase
• UniProt Gene Name: ATP4A

NCBI Description

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for acid production in the stomach.