Typical Testing Data/Standard Curve (for reference only)
Human ATP Binding Cassette Transporter C8 (ABCC8) ELISA Kit | ABCC8 elisa kit
Human ATP Binding Cassette Transporter C8 (ABCC8) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ABCA7. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ABCA7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ABCA7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ABCA7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
Uniprot Description
ABCC8: a multi-pass membrane protein subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release. Associates with Kir6.2. Defects in ABCC8 are a cause of persistent hyperinsulinemic hypoglycemia of infancy (PHHI) [MIM:601820, 256450], and may contribute to non-insulin-dependent diabetes mellitus (NIDDM) in Northern European Caucasians. Two alternatively spliced human isoforms have been described.
Protein type: Transporter; Membrane protein, integral; Transporter, ABC family; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11p15.1
Cellular Component: voltage-gated potassium channel complex; plasma membrane
Molecular Function: ATPase activity, coupled to transmembrane movement of substances; sulfonylurea receptor activity; potassium ion transmembrane transporter activity; ATP binding
Biological Process: synaptic transmission; carbohydrate metabolic process; energy reserve metabolic process; signal transduction; transmembrane transport; regulation of insulin secretion; potassium ion transport
Disease: Hyperinsulinemic Hypoglycemia, Familial, 1; Diabetes Mellitus, Transient Neonatal, 2; Diabetes Mellitus, Permanent Neonatal; Hypoglycemia, Leucine-induced; Diabetes Mellitus, Noninsulin-dependent
Research Articles on ABCC8
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Product Notes
The Human ABCC8 abcc8 (Catalog #AAA2000195) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2000195 ELISA Kit recognizes Human ABCC8. It is sometimes possible for the material contained within the vial of "ATP Binding Cassette Transporter C8 (ABCC8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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