Western Blot (WB)
(Western blot detection of SHP1 in Jurkat,C6,Hela cell lysates using SHP1 Rabbit mAb(1:1000 diluted). Predicted band size:68kDa. Observed band size:68kDa.)
Rabbit SHP1 Monoclonal Antibody | anti-PTPN6 antibody
SHP1 Rabbit mAb
IHC: 1:20
Western Blot (WB)
(Western blot detection of SHP1 in Jurkat,C6,Hela cell lysates using SHP1 Rabbit mAb(1:1000 diluted). Predicted band size:68kDa. Observed band size:68kDa.)
Western Blot (WB)
(Western blot detection of SHP1 in Jurkat,C6,Hela cell lysates using SHP1 Rabbit mAb(1:1000 diluted). Predicted band size:68kDa. Observed band size:68kDa.)
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis. Ref.20
Catalytic activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit structure: Monomer. Interacts with MTUS1
By similarity. Interacts with MILR1 (tyrosine-phosphorylated). Interacts with KIT
By similarity. Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, FCRL3, FCRL4, CD300LF, CDK2 and CD84. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.25 Ref.26 Ref.29
Subcellular location: Cytoplasm. Nucleus. Note: In neurons, translocates into the nucleus after treatment with angiotensin II
By similarity. Shuttles between the cytoplasm and nucleus via its association with PDPK1. Ref.26 Ref.29
Tissue specificity: Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Domain: The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation. Ref.31 Ref.33
Post-translational modification: Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation
By similarity. Phosphorylation at Tyr-564 enhances phosphatase activity. Ref.13 Ref.17
Sequence similarities: Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.Contains 2 SH2 domains.Contains 1 tyrosine-protein phosphatase domain.
Research Articles on PTPN6
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Product Notes
The PTPN6 ptpn6 (Catalog #AAA4753023) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHP1 Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000 IHC: 1:20. Researchers should empirically determine the suitability of the PTPN6 ptpn6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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