Human ATP-binding cassette sub-family F member 1 (ABCF1) ELISA Kit | ABCF1 elisa kit

Human ATP-binding cassette sub-family F member 1 (ABCF1) ELISA Kit

Cat. Num. AAA286770

• Gene Names: ABCF1; ABC27; ABC50
• Reactivity: Human
• Synonyms: ATP-binding cassette sub-family F member 1 (ABCF1); Human ATP-binding cassette sub-family F member 1 (ABCF1) ELISA Kit; DADB-129D20.7; ABC27; ABC50; ATP-binding cassette 50 (TNF-alpha stimulated); ATP-binding cassette; sub-family F; member 1; TNFalpha-inducible ATP-binding protein; ABCF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ABCF1. No significant cross-reactivity or interference between Human ABCF1 and analogues was observed.
• Sequence Length: 845

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ABCF1 elisa kit

This assay employs a two-site sandwich ELISA to quantitate ABCF1 in samples. An antibody specific for ABCF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ABCF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 69354671
• NCBI GeneID: 23
• NCBI Accession #: NP_001020262.1
• NCBI GenBank Nucleotide #: NM_001025091.1
• UniProt Accession #: Q8NE71; O14897; Q69YP6; A2BF75
• Molecular Weight: 91,680 Da
• NCBI Official Full Name: ATP-binding cassette sub-family F member 1 isoform a
• NCBI Official Synonym Full Names: ATP binding cassette subfamily F member 1
• NCBI Official Symbol: ABCF1
• NCBI Official Synonym Symbols: ABC27; ABC50
• NCBI Protein Information: ATP-binding cassette sub-family F member 1
• UniProt Protein Name: ATP-binding cassette sub-family F member 1
• Protein Family: ABC transporter F family
• UniProt Gene Name: ABCF1
• UniProt Synonym Gene Names: ABC50

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq, Jul 2008]

Uniprot Description

Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.