Human ATP binding cassette sub family B member 5 (ABCB5) ELISA Kit | ABCB5 elisa kit

Human ATP binding cassette sub family B member 5 (ABCB5) ELISA Kit

Cat. Num. AAA7228707

• Gene Names: ABCB5; ABCB5beta; EST422562; ABCB5alpha
• Reactivity: Human
• Synonyms: ATP binding cassette sub family B member 5 (ABCB5); Human ATP binding cassette sub family B member 5 (ABCB5) ELISA Kit; ABCB5 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for ABCB5 elisa kit

Intended Uses: This ABCB5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ABCB5. This ELISA kit for research use only!

Principle of the Assay||ABCB5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ABCB5 antibody and an ABCB5-HRP conjugate. The assay sample and buffer are incubated together with ABCB5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABCB5 concentration since ABCB5 from samples and ABCB5-HRP conjugate compete for the anti-ABCB5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ABCB5 from the sample, fewer sites are left to bind ABCB5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCB5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ABCB5 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 255708477
• NCBI GeneID: 340273
• NCBI Accession #: NP_001157413.1
• NCBI GenBank Nucleotide #: NM_001163941.1
• Molecular Weight: 138,641 Da
• NCBI Official Full Name: ATP-binding cassette sub-family B member 5 isoform 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 5
• NCBI Official Symbol: ABCB5
• NCBI Official Synonym Symbols: ABCB5beta; EST422562; ABCB5alpha
• NCBI Protein Information: ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5; ATP-binding cassette protein
• UniProt Protein Name: ATP-binding cassette sub-family B member 5
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB5
• UniProt Entry Name: ABCB5_HUMAN

NCBI Description

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

Uniprot Description

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, ABC family; Transporter

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: regulation of membrane potential; metabolic process; compound eye corneal lens development; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; cell differentiation; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; transmembrane transport; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent