Human ATP-binding cassette sub-family B member 5, ABCB5 ELISA Kit | ABCB5 elisa kit

Human ATP-binding cassette sub-family B member 5, ABCB5 ELISA Kit

Cat. Num. AAA2602687

• Gene Names: ABCB5; ABCB5beta; EST422562; ABCB5alpha
• Reactivity: Human
• Synonyms: ATP-binding cassette sub-family B member 5; ABCB5; Human ATP-binding cassette sub-family B member 5; ABCB5 ELISA Kit; ABCB5 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant ABCB5 concentration.
• Assay Type: Sandwich
• Detection Range: 10 ng/ml-0.156ng/ml
• Sensitivity: 0.05 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for ABCB5 elisa kit

Principle of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ABCB5 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 255708477
• NCBI GeneID: 340273
• NCBI Accession #: NP_001157413.1
• NCBI GenBank Nucleotide #: NM_001163941.1
• UniProt Accession #: Q2M3G0; Q2M3I5; Q5I5Q7; Q5I5Q8; Q6KG50; Q6XFQ5; A4D131; A7BKA4; B5MD19; B7WPL1; F8QQP8; J3KQ04
• Molecular Weight: 89,832 Da
• NCBI Official Full Name: ATP-binding cassette sub-family B member 5 isoform 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 5
• NCBI Official Symbol: ABCB5
• NCBI Official Synonym Symbols: ABCB5beta; EST422562; ABCB5alpha
• NCBI Protein Information: ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5; ATP-binding cassette protein
• UniProt Protein Name: ATP-binding cassette sub-family B member 5
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB5
• UniProt Entry Name: ABCB5_HUMAN

NCBI Description

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

Uniprot Description

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Transporter; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: regulation of membrane potential; metabolic process; compound eye corneal lens development; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; cell differentiation; transmembrane transport; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent