Rabbit anti-Human PARP3 Polyclonal Antibody | anti-PARP3 antibody

PARP3 Polyclonal Antibody

Cat. Num. AAA3012038

• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PARP3; Polyclonal Antibody; PARP3 Polyclonal Antibody; Poly [ADP-ribose] polymerase 3 (EC: 2.4.2.30); PARP-3; hPARP-3; ADP-ribosyltransferase diphtheria toxin-like 3; ARTD3; IRT1; NAD(+) ADP-ribosyltransferase 3; ADPRT-3; Poly[ADP-ribose] synthase 3; pADPRT-3; ADPRT3; ADPRTL3; anti-PARP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PARP3 polyclonal antibody detects endogenous levels of PARP3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 540

• Applicable Applications for anti-PARP3 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant full length Human PARP3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using PARP3 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PARP3 antibody

Poly(ADP-ribose) polymerase-3 (PARP-3) is part of the base excision repair (BER) pathway, catalyzing the poly(ADP-ribosyl)ation of nuclear proteins. Poly(ADP-ribosyl)ation, a post-translational modification following DNA damage, appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-3 is a nuclear, DNA-binding protein, which interacts with PARP-1. PARP-3 is present in actively dividing tissues with highest levels in the kidney, skeletal muscle, liver, heart and spleen. Human PARP-3 maps to chromosome 3p21-3-p21.1, a gene region that undergoes alteration in solid malignant tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 158634478
• NCBI Accession #: NP_001003931.2
• NCBI GenBank Nucleotide #: NP_001003931.2
• UniProt Accession #: Q9Y6F1
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: poly
• UniProt Protein Name: Poly [ADP-ribose] polymerase 3
• Protein Family: Poly [ADP-ribose] polymerase
• UniProt Gene Name: PARP3
• UniProt Synonym Gene Names: ADPRT3; ADPRTL3; PARP-3; hPARP-3; ARTD3; ADPRT-3; pADPRT-3
• UniProt Entry Name: PARP3_HUMAN

Uniprot Description

PARP3: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. May link the DNA damage surveillance network to the mitotic fidelity checkpoint. Negatively influences the G1/S cell cycle progression without interfering with centrosome duplication. Binds DNA. May be involved in the regulation of PRC2 and PRC3 complex-dependent gene silencing. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.2.30; DNA repair, damage; Transferase

Chromosomal Location of Human Ortholog: 3p21.2

Cellular Component: centriole; nucleus

Molecular Function: catalytic activity; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; double-strand break repair; positive regulation of DNA ligation; DNA repair; telomere maintenance