Human AT hook DNA binding motif containing protein 1 (AHDC1) ELISA Kit | AHDC1 elisa kit

Human AT hook DNA binding motif containing protein 1 (AHDC1) ELISA Kit

Cat. Num. AAA7225826

• Gene Names: AHDC1; MRD25
• Reactivity: Human
• Synonyms: AT hook DNA binding motif containing protein 1 (AHDC1); Human AT hook DNA binding motif containing protein 1 (AHDC1) ELISA Kit; AHDC1 elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AHDC1 elisa kit

Intended Uses: This AGTR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AGTR1. This ELISA kit for research use only!

Principle of the Assay: AGTR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AGTR1 antibody and an AGTR1-HRP conjugate. The assay sample and buffer are incubated together with AGTR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGTR1 concentration since AGTR1 from samples and AGTR1-HRP conjugate compete for the anti-AGTR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AGTR1 from the sample, fewer sites are left to bind AGTR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGTR1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AHDC1 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 71274144
• NCBI GeneID: 27245
• NCBI Accession #: NP_001025053.1
• NCBI GenBank Nucleotide #: NM_001029882.3
• Molecular Weight: 168,349 Da
• NCBI Official Full Name: AT-hook DNA-binding motif-containing protein 1
• NCBI Official Synonym Full Names: AT hook, DNA binding motif, containing 1
• NCBI Official Symbol: AHDC1
• NCBI Official Synonym Symbols: MRD25
• NCBI Protein Information: AT-hook DNA-binding motif-containing protein 1
• UniProt Protein Name: AT-hook DNA-binding motif-containing protein 1
• Protein Family: AT-hook DNA-binding motif-containing protein
• UniProt Gene Name: AHDC1
• UniProt Entry Name: AHDC1_HUMAN

NCBI Description

This gene encodes a protein containing two AT-hooks, which likely function in DNA binding. Mutations in this gene were found in individuals with Xia-Gibbs syndrome. [provided by RefSeq, Jun 2014]

Uniprot Description

AHDC1:

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p36.13

Molecular Function: DNA binding

Disease: Xia-gibbs Syndrome