Typical Testing Data/Standard Curve (for reference only)
Mouse DNA topoisomerase 2-alpha (TOP2A) ELISA Kit | TOP2A elisa kit
Mouse DNA topoisomerase 2-alpha (TOP2A) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TOP2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-TOP2A antibody and an TOP2A-HRP conjugate. The assay sample and buffer are incubated together with TOP2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TOP2A concentration since TOP2A from samples and TOP2A-HRP conjugate compete for the anti-TOP2A antibody binding site. Since the number of sites is limited, as more sites are occupied by TOP2A from the sample, fewer sites are left to bind TOP2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TOP2A concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
Uniprot Description
TOP2A: a DNA topoisomerase. Controls of topological states of DNA by transient breakage and rejoining of DNA. Makes double-strand breaks. Four splice variant isoforms have been described.
Protein type: Isomerase; EC 5.99.1.3
Chromosomal Location of Human Ortholog: 17q21-q22
Cellular Component: nucleoplasm; centriole; nuclear chromosome; viral integration complex; protein complex; DNA topoisomerase complex (ATP-hydrolyzing); nucleolus; condensed chromosome; nucleus
Molecular Function: protein C-terminus binding; DNA-dependent ATPase activity; DNA topoisomerase (ATP-hydrolyzing) activity; ubiquitin binding; protein homodimerization activity; histone deacetylase binding; magnesium ion binding; drug binding; protein binding; enzyme binding; DNA binding; protein kinase C binding; protein heterodimerization activity; chromatin binding; DNA bending activity; ATP binding
Biological Process: DNA unwinding during replication; mitotic recombination; DNA topological change; positive regulation of viral genome replication; positive regulation of apoptosis; sister chromatid segregation; rhythmic process; apoptotic chromosome condensation; regulation of circadian rhythm; embryonic cleavage; positive regulation of retroviral genome replication; chromosome segregation; DNA ligation; resolution of meiotic joint molecules as recombinants; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; mitotic cell cycle; response to DNA damage stimulus
Research Articles on TOP2A
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Product Notes
The Mouse TOP2A top2a (Catalog #AAA7239901) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7239901 ELISA Kit recognizes Mouse TOP2A. It is sometimes possible for the material contained within the vial of "DNA topoisomerase 2-alpha (TOP2A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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