Mouse Asparagine Synthetase (ASNS) ELISA Kit | ASNS elisa kit

Mouse Asparagine Synthetase (ASNS) ELISA Kit

Cat. Num. AAA2705227

• Gene Names: ASNS; TS11; ASNSD
• Reactivity: Mouse
• Synonyms: Asparagine Synthetase (ASNS); Mouse Asparagine Synthetase (ASNS) ELISA Kit; Aspartate-Ammonia Ligase; Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; ASNS elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Asparagine Synthetase (ASNS).; No significant cross-reactivity or interference between Asparagine Synthetase (ASNS) and analogues was observed.
• Sequence Length: 478

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.108ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Asparagine Synthetase (ASNS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ASNS elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asparagine Synthetase (ASNS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Asparagine Synthetase (ASNS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Asparagine Synthetase (ASNS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Asparagine Synthetase (ASNS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 533200
• NCBI GeneID: 440
• UniProt Accession #: P08243; P08184; Q15666; Q549T9; Q96HD0; A4D1I8; B4DXZ1; B7ZAA9; D6W5R3; E9PCI3; E9PCX6
• Molecular Weight: 54,818 Da
• NCBI Official Full Name: asparagine synthetase
• NCBI Official Synonym Full Names: asparagine synthetase (glutamine-hydrolyzing)
• NCBI Official Symbol: ASNS
• NCBI Official Synonym Symbols: TS11; ASNSD
• NCBI Protein Information: asparagine synthetase [glutamine-hydrolyzing]
• UniProt Protein Name: Asparagine synthetase [glutamine-hydrolyzing]
• Protein Family: Asparagine--tRNA ligase
• UniProt Gene Name: ASNS
• UniProt Synonym Gene Names: TS11

NCBI Description

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]