Testing Data
(IHC analysis of HDEL tested using a human keratinocyte line, HaCat.Courtesy of Dr. Turksen, Ottawa Research Hospital Institute, Canada.)
Mouse HDEL Monoclonal Antibody
HDEL Antibody: ATTO 488
Reactivity
Drosophilia, Yeast, Saccharomyce, Barnyard grass, Beet, Cotton, Mung bean, Sorghum, Wheat
Applications
Western Blot, Immunofluorescence
Synonyms
HDEL; Monoclonal Antibody; HDEL Antibody: ATTO 488; H-D-E-L (his-asp-glu-leu); endoplasmic reticulum; luminal ER protein retention; KDELR1; Endoplasmic reticulum retention signal; anti-HDEL antibody
Host
Mouse
Reactivity
Drosophilia, Yeast, Saccharomyce, Barnyard grass, Beet, Cotton, Mung bean, Sorghum, Wheat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2E7
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-HDEL antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
1:100-1:1000 (WB), 1:50-1:500 (IF)
Conjugate
ATTO 488
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Testing Data
(IHC analysis of HDEL tested using a human keratinocyte line, HaCat.Courtesy of Dr. Turksen, Ottawa Research Hospital Institute, Canada.)
Testing Data
(IHC analysis of HDEL tested using a human keratinocyte line, HaCat.Courtesy of Dr. Turksen, Ottawa Research Hospital Institute, Canada.)
Related Product Information for anti-HDEL antibody
Background Info: Detects ~78kDa.
Scientific Background: HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
Scientific Background: HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
Product Categories/Family for anti-HDEL antibody
References
1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. Entrez Gene: HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.
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Product Notes
The HDEL (Catalog #AAA802938) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HDEL Antibody: ATTO 488 reacts with Drosophilia, Yeast, Saccharomyce, Barnyard grass, Beet, Cotton, Mung bean, Sorghum, Wheat and may cross-react with other species as described in the data sheet. AAA Biotech's HDEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). 1:100-1:1000 (WB), 1:50-1:500 (IF). Researchers should empirically determine the suitability of the HDEL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDEL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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