Western Blot (WB)
(Western blot analysis of ATMIN in 293 cell lysate with ATMIN antibody at (A) 1 and (B) 2 μg/ml.)
Rabbit ATMIN Polyclonal Antibody | anti-ATMIN antibody
ATMIN Antibody
Gene Names
ATMIN; ASCIZ; ZNF822
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
ATMIN antibody is affinity chromatography purified via peptide column.
Synonyms
ATMIN; Polyclonal Antibody; ATMIN Antibody; ASCIZ; ZNF822; KIAA0431; ATM interactor; Zinc finger protein 822; anti-ATMIN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ATMIN antibody is human, mouse and rat reactive. At least three isoforms of ATMIN are known to exist.
Purity/Purification
ATMIN antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
823
Applicable Applications for anti-ATMIN antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunocytochemistry at 5 mug/mL. For Immunoflorescence start at 2.5 mug/mL.
Conjugate
Unconjugated
Immunogen
ATMIN antibody was raised against a 19 amino acid peptide near the amino terminus of human ATMIN.
Buffer
ATMIN Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ATMIN antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of ATMIN in 293 cell lysate with ATMIN antibody at (A) 1 and (B) 2 μg/ml.)
Western Blot (WB)
(Western blot analysis of ATMIN in 293 cell lysate with ATMIN antibody at (A) 1 and (B) 2 μg/ml.)
Immunocytochemistry (IHC)
(Immunocytochemistry of ATMIN in A431 cells with ATMIN antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of ATMIN in A431 cells with ATMIN antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ATMIN in A431 cells with ATMIN antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ATMIN in A431 cells with ATMIN antibody at 2.5 μg/mL.)
Related Product Information for anti-ATMIN antibody
The ATM/ATR-substrate CHK2-interacting zinc finger protein (ATMIN), also known as ASCIZ, forms DNA damage-induced nuclear foci that contain the DNA repair protein Rad51 (1). ATMIN is also thought to be involved in embryonic development, as an absence of ATMIN causes late-embryonic lethality in mice with a range of organ development defects (2). It also activates the transcription DYNLL1, a light chain of the dynein motor complex and sequence-specific regulator of protein dimerization of numerous targets. DYNLL1 can bind to and inhibit the transcription activation domain of ATMIN, forming a simple dynamic feedback loop for DYNLL1 expression (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 73; 79; 91 kDa
Observed: 72 kDa
Observed: 72 kDa
NCBI Official Full Name
ATM interactor isoform 1
NCBI Official Synonym Full Names
ATM interactor
NCBI Official Symbol
ATMIN
NCBI Official Synonym Symbols
ASCIZ; ZNF822
NCBI Protein Information
ATM interactor; ATM INteracting protein; zinc finger protein 822; ATM/ATR-Substrate Chk2-Interacting Zn++-finger protein; ATM/ATR-substrate CHK2-interacting zinc finger protein; ATM/ATR-substrate CHEK2-interacting zinc finger protein
UniProt Protein Name
ATM interactor
Protein Family
UniProt Gene Name
ATMIN
UniProt Synonym Gene Names
KIAA0431; ZNF822; ASCIZ
UniProt Entry Name
ATMIN_HUMAN
Uniprot Description
ATMIN: Transcription factor. Plays a crucial role in cell survival and RAD51 foci formation in response to methylating DNA damage. Involved in regulating the activity of ATM in the absence of DNA damage. May play a role in stabilizing ATM. Binds to the DYNLL1 promoter and activates its transcription. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: C2H2-type zinc finger protein; DNA repair, damage
Chromosomal Location of Human Ortholog: 16q23.2
Cellular Component: nucleus
Molecular Function: dynein binding; protein binding; metal ion binding
Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; response to DNA damage stimulus
Research Articles on ATMIN
Similar Products
Product Notes
The ATMIN atmin (Catalog #AAA153392) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATMIN Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATMIN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunocytochemistry at 5 mug/mL. For Immunoflorescence start at 2.5 mug/mL. Researchers should empirically determine the suitability of the ATMIN atmin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATMIN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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