Human AREG (Amphiregulin) ELISA Kit | AREG elisa kit

Human AREG (Amphiregulin) ELISA Kit

Cat. Num. AAA7607230

• Gene Names: AREG; AR; SDGF; AREGB; CRDGF
• Reactivity: Human
• Synonyms: AREG (Amphiregulin); Human AREG (Amphiregulin) ELISA Kit; AREG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of. No significant cross-reactivity or interference between and analogues was observed.
• Sequence Length: 252

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: <18.75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AREG elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502199
• NCBI GeneID: 374
• NCBI Accession #: NP_001648.1
• NCBI GenBank Nucleotide #: NM_001657.4
• UniProt Accession #: P15514; Q5U026
• Molecular Weight: 27,895 Da
• NCBI Official Full Name: amphiregulin preproprotein
• NCBI Official Synonym Full Names: amphiregulin
• NCBI Official Symbol: AREG
• NCBI Official Synonym Symbols: AR; SDGF; AREGB; CRDGF
• NCBI Protein Information: amphiregulin
• UniProt Protein Name: Amphiregulin
• Protein Family: Amphiregulin
• UniProt Gene Name: AREG
• UniProt Synonym Gene Names: AR1 PublicationManual assertion based on opinion iniRef.1; CRDGF1 PublicationManual assertion based on opinion iniRef.10

NCBI Description

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

Uniprot Description

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.