Human Amphiregulin(AR) ELISA Kit | AREG elisa kit

Human Amphiregulin(AR) PicoKine ELISA Kit

Cat. Num. AAA177342

• Gene Names: AREG; AR; SDGF; AREGB; CRDGF
• Reactivity: Human
• Synonyms: Amphiregulin(AR); Human Amphiregulin(AR) PicoKine ELISA Kit; Amphiregulin; amphiregulin; A REG; AR; AREG; Colorectum cell derived growth factor; CRDGF; MGC13647; OTTHUMP00000219184; Schwannoma derived growth factor; SDGF; AREG elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Amphiregulin
• Sequence Length: 252

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), saliva and human milk
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 10pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human Amphiregulin(AR) PicoKine ELISA Kit standard curve )

Related Product Information for AREG elisa kit

Principle of the assay: human Amphiregulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Amphiregulin has been precoated onto 96-well plates. Standards(E.coli, S101-K198) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Amphiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Amphiregulin amount of sample captured in plate.
Background: Amphiregulin, also known as AREG, is a protein that in humans is encoded by the AREG gene. The protein encoded by this gene is a member of the epidermal growth factor family. This gene is mapped to 4q13.1. It is an autocrine growth factor as well as a mitogen forastrocytes, Schwann cells, fibroblasts. Amphiregulin is related to toepidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the Epidermal growth factor receptor to promote the growth of normal epithelial cells. It has been found that transgene integration and subsequent expression of Amphiregulin in basal keratinocytes correlated with a psoriasis-like skin phenotype, and its expression was increased in parallel with typical Th2 cytokines. Amphiregulin is also an important paracrine mediator of estrogen function specifically required for puberty-induced ductal elongation but not for any earlier or later developmental stages, and it can enhance resistance to nematodes.

NCBI and Uniprot Product Information

• NCBI GI #: 113754
• NCBI GeneID: 374
• UniProt Accession #: P15514; Q5U026
• Molecular Weight: 27,895 Da
• NCBI Official Full Name: Amphiregulin
• NCBI Official Synonym Full Names: amphiregulin
• NCBI Official Symbol: AREG
• NCBI Official Synonym Symbols: AR; SDGF; AREGB; CRDGF
• NCBI Protein Information: amphiregulin; amphiregulin B; schwannoma-derived growth factor; colorectum cell-derived growth factor
• UniProt Protein Name: Amphiregulin
• Protein Family: Amphiregulin
• UniProt Gene Name: AREG
• UniProt Synonym Gene Names: SDGF; AR; CRDGF
• UniProt Entry Name: AREG_HUMAN

NCBI Description

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

Uniprot Description

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus

Molecular Function: protein binding; growth factor activity; cytokine activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to peptide hormone stimulus; response to cAMP; response to glucocorticoid stimulus; response to estradiol stimulus; glial cell proliferation; cell proliferation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; response to hydrogen peroxide; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; neurite development; positive regulation of DNA replication; positive regulation of phosphorylation