Typical Testing Data/Standard Curve (for reference only)
Human Bullous Pemhpigoid ELISA Kit | BP elisa kit
Human Bullous Pemhpigoid ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: BPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BPP antibody and an BPP-HRP conjugate. The assay sample and buffer are incubated together with BPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BPP concentration since BPP from samples and BPP-HRP conjugate compete for the anti-BPP antibody binding site. Since the number of sites is limited, as more sites are occupied by BPP from the sample, fewer sites are left to bind BPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BPP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of some variants has not been defined. It has been reported that some isoforms are expressed in neural and muscle tissue, anchoring neural intermediate filaments to the actin cytoskeleton, and some isoforms are expressed in epithelial tissue, anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression, mice defective for this gene show skin blistering and neurodegeneration. [provided by RefSeq, Mar 2010]
Uniprot Description
BPAG1 iso3: a protein of the plakin family. Plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. Ten alternatively spliced human isoforms have been reported. BPAG1 has neuronal and muscle isoforms that consist of actin-binding and microtubule-binding domains at either end separated by a plakin domain and many spectrin repeats. Bpag1 isoforms with different N-termini may have differing roles. The plakin domain contains an apparent nuclear localization signal.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Microtubule-binding; Actin-binding
Chromosomal Location of Human Ortholog: 6p12.1
Cellular Component: endoplasmic reticulum membrane; intermediate filament cytoskeleton; focal adhesion; cytoplasmic membrane-bound vesicle; leading edge; integral to membrane; intermediate filament; nuclear envelope; cell cortex; cytosol; Z disc; actin cytoskeleton; microtubule cytoskeleton; hemidesmosome; axon; cytoplasm; basal plasma membrane; basement membrane; nucleus
Molecular Function: protein C-terminus binding; integrin binding; microtubule plus-end binding; protein binding; protein homodimerization activity; calcium ion binding; actin binding
Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; hemidesmosome assembly; response to wounding; cell motility involved in cell locomotion; intermediate filament cytoskeleton organization and biogenesis; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; cell adhesion; maintenance of cell polarity; cell cycle arrest
Research Articles on BP
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Product Notes
The Human BP dst (Catalog #AAA729702) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA729702 ELISA Kit recognizes Human BP. It is sometimes possible for the material contained within the vial of "Bullous Pemhpigoid, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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