Human Apolipoprotein A1 ELISA Kit | APOA1 elisa kit

Human Apolipoprotein A-1 ELISA Kit

Cat. Num. AAA564036

• Gene Names: APOA1; MGC117399
• Reactivity: Human
• Synonyms: Apolipoprotein A1; Human Apolipoprotein A-1 ELISA Kit; Human Apolipoprotein A1; APOA1 elisa kit

• Reactivity: Human

• Samples: Human biological samples
• Assay Type: Sandwich
• Preparation and Storage: Store at 4 degree C.

Related Product Information for APOA1 elisa kit

Intended Uses: The Apo-A1 test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Apo-A1 in human biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use.

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the apolipoprotein A1 (Apo-A1) present in samples reacts with the anti-Apo-A1 antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Apo-A1 antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Apo-A1. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Apo-A1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Apo-A1 in the test sample. The quantity of Apo-A1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Product Categories/Family for APOA1 elisa kit

ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 4960066
• NCBI GeneID: 335
• UniProt Accession #: Q9Y355; P02647; Q6LEJ8; Q8TDB0
• Molecular Weight: 7,433 Da
• NCBI Official Full Name: apolipoprotein A1
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Official Synonym Symbols: MGC117399
• NCBI Protein Information: apolipoprotein A-I; apo-AI; apoA-I; OTTHUMP00000043268; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348
• UniProt Protein Name: Apolipoprotein A1
• Protein Family: Apolipoprotein
• UniProt Entry Name: Q9Y355_HUMAN

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Endoplasmic reticulum; Secreted; Cell development/differentiation; Lipid-binding; Vesicle; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23-q24

Cellular Component: extracellular space; chylomicron; cell surface; endocytic vesicle; endoplasmic reticulum lumen; early endosome; extracellular region; plasma membrane; cytoplasmic vesicle; cytosol; nucleus; vesicle

Molecular Function: identical protein binding; protein binding; enzyme binding; phospholipid transporter activity; lipase inhibitor activity; beta-amyloid binding; chemorepellent activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; high-density lipoprotein binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding

Biological Process: phototransduction, visible light; blood vessel endothelial cell migration; negative chemotaxis; cellular lipid metabolic process; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; negative regulation of interleukin-1 beta secretion; sequestering of lipid; platelet degranulation; phospholipid efflux; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; retinoid metabolic process; transmembrane transport; response to nutrient; phospholipid homeostasis; integrin-mediated signaling pathway; response to drug; receptor-mediated endocytosis; cholesterol metabolic process; platelet activation; positive regulation of fatty acid biosynthetic process; organ regeneration; regulation of protein amino acid phosphorylation; cholesterol transport; regulation of Cdc42 protein signal transduction; negative regulation of heterotypic cell-cell adhesion; protein amino acid oxidation; protein stabilization; adrenal gland development; positive regulation of hydrolase activity; neurite regeneration; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; cholesterol efflux; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; cholesterol homeostasis; reverse cholesterol transport; response to estrogen stimulus; lipoprotein biosynthetic process; peptidyl-methionine modification; endothelial cell proliferation; negative regulation of inflammatory response; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; blood coagulation

Disease: Hypoalphalipoproteinemia, Primary; Amyloidosis, Familial Visceral