Typical Testing Data/Standard Curve (for reference only)
Human Anti-Mullerian Hormone (AMH) ELISA Kit | AMH elisa kit
Anti-Mullerian Hormone (AMH) ELISA
Reactivity
Human
Applications
ELISA
Synonyms
Anti-Mullerian Hormone (AMH); Anti-Mullerian Hormone (AMH) ELISA; AMH ELISA; AMH elisa kit
Reactivity
Human
Applicable Applications for AMH elisa kit
ELISA (EIA)
Samples
Serum or plasma (edta or lithium heparin plasma)
Assay Type
Sandwich
Detection Range
0.062 ng/mL - 20.0 ng/mL
Sensitivity
0.044 ng/mL
Intra-assay Precision
The within-assay variability was determined by measuring each sample 10 times per run (n = 10)
Inter-assay Precision
The between-assay variability was determined by measuring each sample 10 times per run for 3 days (n = 30)
Preparation and Storage
Store at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AMH elisa kit
Intended Uses: The AMH ELISA is an enzyme immunoassay for the measurement of Anti-Müllerian Hormone (AMH) in serum or plasma (EDTA or lithium heparin plasma). For research use only -
Principle of the Assay: The AMH ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal (mouse) antibody directed towards a unique antigenic site of the AMH molecule. During the first incubation, the AMH in the added sample bind to the immobilized antibody. The simultaneously added enzyme conjugate, which contains an AMH antibody conjugated to horseradish peroxidase, binds to the AMH forming a sandwich complex. After a washing step to remove all unbound substances, the solid phase is incubated with the substrate solution. The colorimetric reaction is stopped by addition of stop solution, and optical density (OD) of the resulting yellow product is measured. The intensity of colour is proportional to the concentration of the analyte in the sample. A standard curve is constructed by plotting OD values against concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
Principle of the Assay: The AMH ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal (mouse) antibody directed towards a unique antigenic site of the AMH molecule. During the first incubation, the AMH in the added sample bind to the immobilized antibody. The simultaneously added enzyme conjugate, which contains an AMH antibody conjugated to horseradish peroxidase, binds to the AMH forming a sandwich complex. After a washing step to remove all unbound substances, the solid phase is incubated with the substrate solution. The colorimetric reaction is stopped by addition of stop solution, and optical density (OD) of the resulting yellow product is measured. The intensity of colour is proportional to the concentration of the analyte in the sample. A standard curve is constructed by plotting OD values against concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
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Product Notes
The Human AMH (Catalog #AAA490389) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA490389 ELISA Kit recognizes Human AMH. AAA Biotech's Anti-Mullerian Hormone (AMH) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the AMH for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Anti-Mullerian Hormone (AMH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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