Rabbit anti-Human, Mouse PACT Polyclonal Antibody | anti-PACT antibody

PACT Rabbit Polyclonal Antibody

Cat. Num. AAA588284

• Gene Names: RBBP6; PACT; MY038; P2P-R; RBQ-1; SNAMA
• Reactivity: Human, Mouse
• Applications: Immunoprecipitation, Western Blot, Immunofluorescence
• Synonyms: PACT; Polyclonal Antibody; PACT Rabbit Polyclonal Antibody; Interferon-inducible double stranded RNA-dependent protein kinase activator A; anti-PACT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody recognizes the human PACT and the mouse homologous RAX.
• Form/Format: Lyophilized immunogen affinity purified in PBS pH7.4
• Sequence Length: 1625

• Applicable Applications for anti-PACT antibody: Immunoprecipitation (IP), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: If reconstituted in 50 muL: IP: 10pg; WB: 1:1000-1:3000; IF: 1:500.
• Preservative: None
• Preparation and Storage: Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.; To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).; For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of PACT expression in Iysates of Jurkat cells, astrocytes and 2931 cells. GAPDH was used as a loading control (anti-PACT was used at 1:2000).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of PACT.PACT was immunoprecipitated from 293T cells with 10mug of the anti-PACT antibody.)

Related Product Information for anti-PACT antibody

The Interferon-inducible double stranded RNA-dependent protein kinase activator, also known as PKR-associated protein X (RAX in mouse) or PACT (in human) is a cellular protein that heterodimerizes with PKR through its double-stranded RNA binding proteins (dsRBDs). PKR is an interferon (IFN)-induced serine-threonine protein kinase that plays a central role in IFN's antiviral and antiproliferative activities. In the absence of viral infections, PACT activates PKR in response to diverse stress signals as serum starvation and treatments with arsenite or peroxide, resulting in the induction of the apoptotic cascade. PACT is expressed in most cell types at a very low abundance, and overexpression of PACT causes PKR activation, leading to elF2a phosphorylation. PACT has been shown to interact with another DRBM-containing protein, TRBP (TAR RNA binding protein), and form a triple complex with Dicer to facilitate the production of siRNA.

References

1. Daher A-TRBP control of PACT-induced phosphorylation of protein kinase R is reversed by stress.

NCBI and Uniprot Product Information

• NCBI GI #: 37543028
• NCBI GeneID: 5930
• Molecular Weight: 197,287 Da
• NCBI Official Full Name: p53-associated cellular protein PACT
• NCBI Official Synonym Full Names: RB binding protein 6, ubiquitin ligase
• NCBI Official Symbol: RBBP6
• NCBI Official Synonym Symbols: PACT; MY038; P2P-R; RBQ-1; SNAMA
• NCBI Protein Information: E3 ubiquitin-protein ligase RBBP6
• UniProt Protein Name: E3 ubiquitin-protein ligase RBBP6
• UniProt Gene Name: RBBP6
• UniProt Synonym Gene Names: P2PR; PACT; RBQ1; RBQ-1

NCBI Description

The retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome (PubMed:18851979). May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth (). Regulates DNA-replication and the stability of chromosomal common fragile sites (CFSs) in a ZBTB38- and MCM10-dependent manner. Controls ZBTB38 protein stability and abundance via ubiquitination and proteasomal degradation, and ZBTB38 in turn negatively regulates the expression of MCM10 which plays an important role in DNA-replication (PubMed:24726359).