Typical Testing Data/Standard Curve (for reference only)
Monkey Oxidosqualene Cyclase ELISA Kit | OSC elisa kit
Monkey Oxidosqualene Cyclase ELISA Kit
Reactivity
Monkey
Synonyms
Oxidosqualene Cyclase; Monkey Oxidosqualene Cyclase ELISA Kit; OSC elisa kit
Reactivity
Monkey
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100ng/mL
Sensitivity
1.0ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for OSC elisa kit
Intended Uses: This OSC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey OSC. This ELISA kit for research use only!
Principle of the Assay: OSC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OSC antibody and an OSC-HRP conjugate. The assay sample and buffer are incubated together with OSC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OSC concentration since OSC from samples and OSC-HRP conjugate compete for the anti-OSC antibody binding site. Since the number of sites is limited, as more sites are occupied by OSC from the sample, fewer sites are left to bind OSC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OSC concentration in each sample is interpolated from this standard curve.
Principle of the Assay: OSC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OSC antibody and an OSC-HRP conjugate. The assay sample and buffer are incubated together with OSC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OSC concentration since OSC from samples and OSC-HRP conjugate compete for the anti-OSC antibody binding site. Since the number of sites is limited, as more sites are occupied by OSC from the sample, fewer sites are left to bind OSC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OSC concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
83,301 Da
NCBI Official Full Name
oxidosqualene cyclase
NCBI Official Synonym Full Names
lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
NCBI Official Symbol
Lss
NCBI Protein Information
lanosterol synthase; OSC; oxidosqualene--lanosterol cyclase; 2,3-epoxysqualene--lanosterol cyclase; 2,3-oxidosqualene: lanosterol cyclase
UniProt Protein Name
Lanosterol synthase
UniProt Gene Name
Lss
UniProt Synonym Gene Names
Osc; OSC
UniProt Entry Name
ERG7_RAT
NCBI Description
enzyme that catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol [RGD, Feb 2006]
Uniprot Description
LSS: Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. Belongs to the terpene cyclase/mutase family.
Protein type: EC 5.4.99.7; Lipid Metabolism - steroid biosynthesis; Isomerase
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane; lipid particle
Molecular Function: lanosterol synthase activity; intramolecular transferase activity
Biological Process: metabolic process; sterol metabolic process; cholesterol biosynthetic process
Research Articles on OSC
Similar Products
Product Notes
The Monkey OSC lss (Catalog #AAA743844) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA743844 ELISA Kit recognizes Monkey OSC. It is sometimes possible for the material contained within the vial of "Oxidosqualene Cyclase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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