Human Angiotensin I Converting Enzyme (ACE) ELISA Kit | ACE elisa kit
Human Angiotensin I Converting Enzyme (ACE) ELISA Kit
Gene Names
AGTR2; AT2; ATGR2; MRX88
Reactivity
Human
Synonyms
Angiotensin I Converting Enzyme (ACE); Human Angiotensin I Converting Enzyme (ACE) ELISA Kit; ACE1; CD143; DCP; DCP1; MGC26566; MVCD3; CD143 antigen; angiotensin I converting enzyme 1; angiotensin converting enzyme; somatic isoform; carboxycathepsin; dipeptidyl carboxypeptidase 1; kininase II; pep; ACE elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ACE. No significant cross-reactivity or interference between Human ACE and analogues was observed.
Sequence Length
363
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
6.25 ng/mL - 400 ng/mL
Sensitivity
3.12 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ACE elisa kit
Intended Uses: For the quantitative detection of Human Angiotensin I Converting Enzyme (ACE) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ACE in Human serum, plasma. An antibody specific for ACE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ACE in Human serum, plasma. An antibody specific for ACE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACE bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,184 Da
NCBI Official Full Name
type-2 angiotensin II receptor
NCBI Official Synonym Full Names
angiotensin II receptor type 2
NCBI Official Symbol
AGTR2
NCBI Official Synonym Symbols
AT2; ATGR2; MRX88
NCBI Protein Information
type-2 angiotensin II receptor
UniProt Protein Name
Type-2 angiotensin II receptor
Protein Family
UniProt Gene Name
AGTR2
UniProt Synonym Gene Names
AT2
NCBI Description
The protein encoded by this gene belongs to the G-protein coupled receptor 1 family, and functions as a receptor for angiotensin II. It is an intergral membrane protein that is highly expressed in fetus, but scantily in adult tissues, except brain, adrenal medulla, and atretic ovary. This receptor has been shown to mediate programmed cell death and this apoptotic function may play an important role in developmental biology and pathophysiology. Mutations in this gene are been associated with X-linked cognitive disability. [provided by RefSeq, Jan 2010]
Uniprot Description
Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
Research Articles on ACE
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Product Notes
The Human ACE agtr2 (Catalog #AAA286788) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA286788 ELISA Kit recognizes Human ACE. It is sometimes possible for the material contained within the vial of "Angiotensin I Converting Enzyme (ACE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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