Human AMP deaminase 1 (AMPD1) ELISA Kit | AMPD1 elisa kit
Human AMP deaminase 1 (AMPD1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AMPD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-AMPD1 antibody and an AMPD1-HRP conjugate. The assay sample and buffer are incubated together with AMPD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMPD1 concentration since AMPD1 from samples and AMPD1-HRP conjugate compete for the anti-AMPD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AMPD1 from the sample, fewer sites are left to bind AMPD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPD1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]
Uniprot Description
AMPD1: AMP deaminase plays a critical role in energy metabolism. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM). AMPDDM is a metabolic disorder resulting in exercise-related myopathy. It is characterized by exercise-induced muscle aches, cramps, and early fatigue. Belongs to the adenosine and AMP deaminases family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.5.4.6; Hydrolase; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 1p13
Cellular Component: cytosol
Molecular Function: metal ion binding; AMP deaminase activity; myosin heavy chain binding
Biological Process: response to organic substance; IMP salvage; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; purine salvage
Disease: Myopathy Due To Myoadenylate Deaminase Deficiency
