Testing Data
EZ-Safe DNA Reagent
EZ-Safe DNA Stain Buffer
Synonyms
EZ-Safe DNA; EZ-Safe DNA Stain Buffer; EZ Safe-Stain DNA Loading Buffer; EZ Safe-Stain DNA Stain Buffer; EZ-Safe DNA reagent
Application Notes
Visualization of DNA bands as they separate during agarose gel electrophoresis using blue light;
Isolation of DNA fragments for subclonning without introducing mutations normally caused by EB and ultraviolet.
Note
1) The thickness of gel could affect the gel sensitivity.
2) EZ Safe-Stain allows visualization of DNA in the agarose gel under blue light. This eliminates the need for exposure to UV light, which can nick and damage DNA. The intact DNA fragments purified from agarose gel can increase the efficiency of subsequent molecular biology manipulations such as cloning, sequencing, transformation and transcription.
2) EZ Safe-Stain allows visualization of DNA in the agarose gel under blue light. This eliminates the need for exposure to UV light, which can nick and damage DNA. The intact DNA fragments purified from agarose gel can increase the efficiency of subsequent molecular biology manipulations such as cloning, sequencing, transformation and transcription.
Protocol
1. Prepare agarose gel in 1x TBE or 1x TAE (or use a precast agarose gel).
2. Mix well the 6x EZ Safe-Stain DNA loading buffer prior to use.
3. Add 1x EZ Safe-Stain DNA loading buffer to DNA samples or DNA ladders, and then proceed directly to sample loading.
4. Run the agarose gel.
5. Monitor the DNA migration under blue light or with a blue light monitor.
6. Document the image under the UV light. We recommend not using UV light if the DNA fragments are to be used for subsequent cloning or sequencing.
2. Mix well the 6x EZ Safe-Stain DNA loading buffer prior to use.
3. Add 1x EZ Safe-Stain DNA loading buffer to DNA samples or DNA ladders, and then proceed directly to sample loading.
4. Run the agarose gel.
5. Monitor the DNA migration under blue light or with a blue light monitor.
6. Document the image under the UV light. We recommend not using UV light if the DNA fragments are to be used for subsequent cloning or sequencing.
Contents
1 ml (6x) in DNA loading buffer.
Preparation and Storage
Store at 4 degree C in dark, stable for one year from the date of shipment. For long term storage: -20 degree C.
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for EZ-Safe DNA reagent
Description: EZ Safe-Stain is an environment safe non-EB nucleic acid stain used for detecting double-stranded DNA in agarose gels. EZ Safe-Stain is supplied in 6x DNA loading buffer which contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G).
EZ Safe-Stain is as sensitive as EB; compared to EB known as a strong mutagen, EZ Safe-Stain is a non-mutagenic stain. EZ Safe-Stain is an environment-friendly non-EB stain for detecting nucleic acid in agarose gels, especially when isolating DNA fragment for subclonning.
EZ Safe-Stain is as sensitive as EB; compared to EB known as a strong mutagen, EZ Safe-Stain is a non-mutagenic stain. EZ Safe-Stain is an environment-friendly non-EB stain for detecting nucleic acid in agarose gels, especially when isolating DNA fragment for subclonning.
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Product Notes
The EZ-Safe DNA (Catalog #AAA433668) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. Visualization of DNA bands as they separate during agarose gel electrophoresis using blue light; Isolation of DNA fragments for subclonning without introducing mutations normally caused by EB and ultraviolet. Researchers should empirically determine the suitability of the EZ-Safe DNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EZ-Safe DNA, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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