Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) Standard Curve.)
Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit | MGAT4C elisa kit
Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit
Gene Names
MGAT4C; GNTIVH; HGNT-IV-H
Reactivity
Human
Synonyms
Alpha-1; 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C); Human Alpha-1; 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit; MGAT4C; GNTIVH; HGNT-IV-H; UDP-N-acetylglucosamine:a-1; 3-D-mannoside beta-1; 4-N-acetylglucosaminyltransferase IV; 4-N-acetylglucosaminyltransferase IV-homolog; MGAT4C elisa kit
Reactivity
Human
Specificity
Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit has high sensitivity and excellent specificity for detection of Human MGAT4C. No significant cross-reactivity or interference between Human MGAT4C and analogues was observed.
Sequence Length
478
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) Standard Curve.)
Related Product Information for MGAT4C elisa kit
Description: This Human Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C) ELISA Kit employs a two-site sandwich ELISA to quantitate MGAT4C in samples. An antibody specific for MGAT4C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MGAT4C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGAT4C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGAT4C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MGAT4C (MGAT4 Family Member C) is a Protein Coding gene. Diseases associated with MGAT4C include Mannosidosis, Beta and Muscular Dystrophy, Congenital. Among its related pathways are Transport to the Golgi and subsequent modification and Metabolism of proteins. GO annotations related to this gene include transferase activity, transferring hexosyl groups and alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity. An important paralog of this gene is MGAT4A. Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains (By similarity). Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage (GnT-VI activity).
Background: MGAT4C (MGAT4 Family Member C) is a Protein Coding gene. Diseases associated with MGAT4C include Mannosidosis, Beta and Muscular Dystrophy, Congenital. Among its related pathways are Transport to the Golgi and subsequent modification and Metabolism of proteins. GO annotations related to this gene include transferase activity, transferring hexosyl groups and alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity. An important paralog of this gene is MGAT4A. Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains (By similarity). Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage (GnT-VI activity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C isoform c
NCBI Official Synonym Full Names
MGAT4 family member C
NCBI Official Symbol
MGAT4C
NCBI Official Synonym Symbols
GNTIVH; HGNT-IV-H
NCBI Protein Information
alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
UniProt Protein Name
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C
UniProt Gene Name
MGAT4C
UniProt Synonym Gene Names
hGnT-IV-H; GlcNAc-T IVc; GnT-IVc; N-acetylglucosaminyltransferase IVc
UniProt Entry Name
MGT4C_HUMAN
Uniprot Description
MGAT4C: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N- linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity). Belongs to the glycosyltransferase 54 family.
Protein type: Transferase; EC 2.4.1.145; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12q21
Cellular Component: Golgi membrane; integral to membrane
Molecular Function: alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity; metal ion binding
Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Research Articles on MGAT4C
Similar Products
Product Notes
The Human MGAT4C mgat4c (Catalog #AAA9715296) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715296 ELISA Kit recognizes Human MGAT4C. It is sometimes possible for the material contained within the vial of "Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C (MGAT4C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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