Typical Testing Data/Standard Curve (for reference only)
Human Alpha 1, 3 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase A (MGAT4A) ELISA Kit | MGAT4A elisa kit
Human Alpha 1, 3 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase A (MGAT4A) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MGAT4A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MGAT4A antibody and an MGAT4A-HRP conjugate. The assay sample and buffer are incubated together with MGAT4A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MGAT4A concentration since MGAT4A from samples and MGAT4A-HRP conjugate compete for the anti-MGAT4A antibody binding site. Since the number of sites is limited, as more sites are occupied by MGAT4A from the sample, fewer sites are left to bind MGAT4A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MGAT4A concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq, Jul 2008]
Uniprot Description
MGAT4A: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N- linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells. Belongs to the glycosyltransferase 54 family.
Protein type: Transferase; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; EC 2.4.1.145
Chromosomal Location of Human Ortholog: 2q12
Cellular Component: Golgi membrane; integral to membrane
Molecular Function: alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity; metal ion binding
Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; N-glycan processing; post-translational protein modification
Research Articles on MGAT4A
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Product Notes
The Human MGAT4A mgat4a (Catalog #AAA7212077) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7212077 ELISA Kit recognizes Human MGAT4A. It is sometimes possible for the material contained within the vial of "Alpha 1, 3 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase A (MGAT4A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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