Rabbit MGAT4A Polyclonal Antibody | anti-MGAT4A antibody

MGAT4A Antibody (N-Term)

Cat. Num. AAA9217207

• Gene Names: MGAT4A; GNT-IV; GnT-4a; GNT-IVA
• Reactivity: Mouse; Predicted Reactivity: Chicken
• Applications: Western Blot, ELISA
• Synonyms: MGAT4A; Polyclonal Antibody; MGAT4A Antibody (N-Term); Alpha-1;3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A; 2.4.1.145; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVa; GlcNAc-T IVa; GnT-IVa; N-acetylglucosaminyltransferase IVa; UDP-N-acetylglucosamine: alpha-1;3-D-mannoside beta-1;4-N-acetylglucosaminyltransferase IVa; Alpha-1;3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form; anti-MGAT4A antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted Reactivity: Chicken
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This MGAT4A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 70-102 amino acids from human MGAT4A.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 423

• Applicable Applications for anti-MGAT4A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB:~~1:2000
• Subtitle: Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Alpha-1,3-mannosyl-glycoprotein 4-beta-N- acetylglucosaminyltransferase A: Golgi apparatus membrane; Single-pass type II membrane protein.
• Tissue Location: Expressed in pancreas, spleen, thymus, prostate, small intestine, peripheral blood leukocytes and lymph node. Strongly overexpressed in choriocarcinoma cancer cell lines Down-regulated in pancreatic cancer.
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-MGAT4A Antibody (N-Term) at 1:2000 dilution + mouse kidney lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MGAT4A antibody

Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N- linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells.

Product Categories/Family for anti-MGAT4A antibody

Primary Antibodies; Metabolism; Signal Transduction

References

Yoshida A.,et al.Glycobiology 9:303-310 (1999). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Hillier L.W.,et al.Nature 434:724-731 (2005). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Minowa M.T.,et al.Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 237512959
• NCBI GeneID: 11320
• NCBI Accession #: NP_001153626.1
• NCBI GenBank Nucleotide #: NM_001160154.1
• UniProt Accession #: Q9UM21; Q53S97; Q86Z15; B4E2R6; D3DVH6; E9PEN2
• Molecular Weight: 61544 Da
• NCBI Official Full Name: alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform 2
• NCBI Official Synonym Full Names: mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
• NCBI Official Symbol: MGAT4A
• NCBI Official Synonym Symbols: GNT-IV; GnT-4a; GNT-IVA
• NCBI Protein Information: alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
• UniProt Protein Name: Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A
• Protein Family: Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• UniProt Gene Name: MGAT4A
• UniProt Synonym Gene Names: GlcNAc-T IVa; GnT-IVa; N-acetylglucosaminyltransferase IVa
• UniProt Entry Name: MGT4A_HUMAN

NCBI Description

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

MGAT4A: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N- linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells. Belongs to the glycosyltransferase 54 family.

Protein type: Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.145; Transferase

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity; metal ion binding

Biological Process: N-glycan processing