Typical Testing Data/Standard Curve (for reference only)
Human Afamin (AFM) ELISA Kit | AFM elisa kit
Human Afamin (AFM) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AFM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AFM antibody and an AFM-HRP conjugate. The assay sample and buffer are incubated together with AFM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AFM concentration since AFM from samples and AFM-HRP conjugate compete for the anti-AFM antibody binding site. Since the number of sites is limited, as more sites are occupied by AFM from the sample, fewer sites are left to bind AFM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AFM concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. [provided by RefSeq, Jul 2008]
Uniprot Description
A-albumin: Vitamin E binding protein. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the regulation and transport of vitamin E at the blood-brain barrier. Belongs to the ALB/AFP/VDB family.
Protein type: Secreted, signal peptide; Secreted; Carrier
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: extracellular space; extracellular region
Molecular Function: vitamin E binding
Biological Process: vitamin transport
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Product Notes
The Human AFM afm (Catalog #AAA7217736) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7217736 ELISA Kit recognizes Human AFM. It is sometimes possible for the material contained within the vial of "Afamin (AFM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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