Human Adipocyte Plasma Membrane Associated Protein (APMAP) ELISA Kit | APMAP elisa kit

Human Adipocyte Plasma Membrane Associated Protein (APMAP) ELISA Kit

Cat. Num. AAA455083

• Gene Names: APMAP; BSCv; C20orf3
• Reactivity: Human
• Synonyms: Adipocyte Plasma Membrane Associated Protein (APMAP); Human Adipocyte Plasma Membrane Associated Protein (APMAP) ELISA Kit; APMAP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of APMAP. No significant cross-reactivity or interference between APMAP and analogues was observed.
• Sequence Length: 416

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.066ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APMAP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APMAP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APMAP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of APMAP in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APMAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APMAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APMAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APMAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 24308201
• NCBI GeneID: 57136
• NCBI Accession #: NP_065392.1
• NCBI GenBank Nucleotide #: NM_020531.2
• UniProt Accession #: Q9HDC9; Q6UVZ8; Q9GZS8; Q9NUB2; A8K514; B4DXG1
• Molecular Weight: 32,161 Da
• NCBI Official Full Name: adipocyte plasma membrane-associated protein
• NCBI Official Synonym Full Names: adipocyte plasma membrane associated protein
• NCBI Official Symbol: APMAP
• NCBI Official Synonym Symbols: BSCv; C20orf3
• NCBI Protein Information: adipocyte plasma membrane-associated protein
• UniProt Protein Name: Adipocyte plasma membrane-associated protein
• Protein Family: Adipocyte plasma membrane-associated protein
• UniProt Gene Name: APMAP
• UniProt Synonym Gene Names: C20orf3

Uniprot Description

Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role in adipocyte differentiation.