Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Adipocyte enhancer-binding protein 1 (AEBP1) Standard Curve.)
Human Adipocyte Enhancer-Binding Protein 1 (AEBP1) ELISA Kit | AEBP1 elisa kit
Human Adipocyte Enhancer-Binding Protein 1 (AEBP1) ELISA Kit
Gene Names
AEBP1; ACLP
Reactivity
Human
Synonyms
Adipocyte Enhancer-Binding Protein 1 (AEBP1); Human Adipocyte Enhancer-Binding Protein 1 (AEBP1) ELISA Kit; AEBP1; ACLP; FLJ33612; AE-binding protein 1; adipocyte enhancer binding protein 1; aortic carboxypeptidase-like protein; AEBP1 elisa kit
Reactivity
Human
Specificity
Human Adipocyte enhancer-binding protein 1 (AEBP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human AEBP1. No significant cross-reactivity or interference between Human AEBP1 and analogues was observed.
Sequence Length
1158
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Adipocyte enhancer-binding protein 1 (AEBP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Adipocyte enhancer-binding protein 1 (AEBP1) Standard Curve.)
Related Product Information for AEBP1 elisa kit
Description: This Human Adipocyte enhancer-binding protein 1 (AEBP1) ELISA Kit employs a two-site sandwich ELISA to quantitate AEBP1 in samples. An antibody specific for AEBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AEBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AEBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AEBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: AEBP1 binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. Adipocyte-enhancer binding protein 1 may play a role in differentiated vascular smooth muscle cells. The deduced 1,158-amino acid ACLP protein contains a putative signal peptide; an 11-residue lys/pro-rich motif repeated 4 times in the N terminus; a domain with 30% identity to the slime mold adhesion protein discoidin I; and a C-terminal 500-amino acid domain with 39% identity to carboxypeptidase E (CPE).
Background: AEBP1 binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. Adipocyte-enhancer binding protein 1 may play a role in differentiated vascular smooth muscle cells. The deduced 1,158-amino acid ACLP protein contains a putative signal peptide; an 11-residue lys/pro-rich motif repeated 4 times in the N terminus; a domain with 30% identity to the slime mold adhesion protein discoidin I; and a C-terminal 500-amino acid domain with 39% identity to carboxypeptidase E (CPE).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
adipocyte enhancer-binding protein 1
NCBI Official Synonym Full Names
AE binding protein 1
NCBI Official Symbol
AEBP1
NCBI Official Synonym Symbols
ACLP
NCBI Protein Information
adipocyte enhancer-binding protein 1
UniProt Protein Name
Adipocyte enhancer-binding protein 1
Protein Family
UniProt Gene Name
AEBP1
UniProt Synonym Gene Names
ACLP; AE-binding protein 1
NCBI Description
This gene encodes a member of carboxypeptidase A protein family. The encoded protein may function as a transcriptional repressor and play a role in adipogenesis and smooth muscle cell differentiation. Studies in mice suggest that this gene functions in wound healing and abdominal wall development. Overexpression of this gene is associated with glioblastoma. [provided by RefSeq, May 2013]
Uniprot Description
Isoform 1: As a positive regulator of collagen fibrillogenesis, it is probably involved in the organization and remodeling of the extracellualr matrix.
Research Articles on AEBP1
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Product Notes
The Human AEBP1 aebp1 (Catalog #AAA9714618) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714618 ELISA Kit recognizes Human AEBP1. It is sometimes possible for the material contained within the vial of "Adipocyte Enhancer-Binding Protein 1 (AEBP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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